Production Of Extracellular Vesicles In Single-cell Suspension Using Chemically-defined Cell Culture Media

  • Published: Mar 28, 2019
  • Earliest Priority: Sep 21 2017
  • Family: 2
  • Cited Works: 2
  • Cited by: 0
  • Cites: 7
  • Additional Info: Cited Works Full text
Abstract

Described herein are methods for the production of extracellular vesicles comprising culturing a population of producer cells in single-cell suspension, wherein the cells are cultured in chemically-defined culture medium, wherein the culture medium lacks animal- derived serum and animal-derived components; and obtaining from the cell culture an extracellular vesicle preparation comprising extracellular vesicles. In certain embodiments, the methods comprise perfusion culturing methods, including single-cell perfusion culturing methods and batch-refeed culturing methods. The methods described herein are a significant improvement over the state of the art and fulfills an unmet need in the field of extracellular vesicle manufacturing and quality control.


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Document History
  • Publication: Mar 28, 2019
  • Application: Sep 20, 2018
    WO US 2018/0052074 W
  • Priority: May 7, 2018
    US US 201862668217 P
  • Priority: Sep 21, 2017
    US US 201762561206 P

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