Methods For Amplification And Sequencing Using Thermostable Tthprimpol

Abstract

The present invention is directed to methods for replicating, amplifying, and sequencing of nucleic acids using the thermostable, bifunctional replicase "TthPrimPol" from Thermus thermophilus HB27. The TthPrimPol enzyme is extremely tolerant to alterations of the nucleotides of the template nucleic acid. Therefore, in one aspect the invention discloses methods for replicating, amplifying and sequencing of damaged polynucleotide templates.


Claims
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Document History
  • Publication: Sep 18, 2014
  • Application: Mar 14, 2014
    WO EP 2014055158 W
  • Priority: Mar 15, 2013
    EP EP 13159629 A

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