Method Of Preparing Polypeptides For Cryo-transmission Electron Microscopy (cryo-em) And Structure Determination Using Dna Lattices

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Abstract

The present invention relates to method for preparing a substance for cryo-transmission electron microscopy (cryo-EM). The present invention also relates to a method for determining the three-dimensional structure of a substance using cryo-EM. The substance is a polypeptide incorporated in a DNA/RNA lattice. The polypeptide may comprise a ligand and may be linked to the DNA nanostructure via tags such as a poly histidine tag.


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Document History
  • Publication: Feb 3, 2011
  • Application: Jul 23, 2010
    WO GB 2010001408 W
  • Priority: Jul 31, 2009
    GB GB 0913433 A

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