Novel Rna Interference Methods Using Dna-rna Duplex Constructs

  • Published: Apr 16, 2009
  • Earliest Priority: Oct 11 2007
  • Family: 4
  • Cited Works: 0
  • Cited by: 0
  • Cites: 2
  • Additional Info: Full text Published

The present invention provides novel compositions and. methods for suppressing the function or activity of a targeted gene through a novel intracellular piRNA-mediated RNAi mechanism, using RNA-DNA duplex constructs. The invention further provides novel methods and compositions for generating or producing RNA-DNA duplex agents, whose quantity is high enough to be used for the invention's gene silencing transfection and possibly in therapeutics applications. This improved RNA-polymerase chain reaction (RNA-PCR) method utilizes thermocycling steps of promoter- linked DNA or RNA template synthesis, in ?itro transcription and then reverse transcription to bring up the amount of RNA-DNA duplexes up to two thousand folds within one round of the above procedure for using in D -RNAi- directed gene silencing.


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