Shrna-mediated Inhibition Of Expression Of Alpha-1. 6-fucosyltransferase


The current invention comprises a method for producing a heterologous polypeptide with a reduced degree of fucose modification in a mammalian cell by cultivating the mammalian cell under conditions suitable for the expression of said heterologous polypeptide, and recovering the heterologous polypeptide from the mammalian cell or the culture, wherein in said mammalian cell the enzymatic activity of al,6-fucosyltransferase is reduced by means of an shRNA directed against al,6-fucosyltransferase mRNA.

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Document History
  • Publication: Jul 3, 2008
  • Application: Dec 19, 2007
    WO EP 2007011160 W
  • Priority: Dec 22, 2006
    EP EP 06026653 A

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