Method Of Screening Multiply Transformed Cells Using Bicistronic Expression Of Fluorescent Proteins

Abstract

A method of screening multiply transformed/transfected cells to identify those cells expressing at least two peptides or proteins of interest. The method comprising: 1. Simultaneously or sequentially transforming a cell with at least two different expression cassettes in which the gene of interest is linked via an IRES to a fluorescent marker gene. Each marker gene is different. 2. Providing conditions in which expression of the genes will occur. 3. Identifying cells expressing proteins by detecting the different fluorescent signals.


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Document History
  • Publication: May 31, 2001
  • Application: Nov 24, 2000
    WO AU 1936/000014 W
  • Priority: Nov 24, 1999
    AU AU PQ422399 A

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