Method Of Screening Multiply Transformed Cells Using Bicistronic Expression Of Fluorescent Proteins


A method of screening multiply transformed/transfected cells to identify those cells expressing at least two peptides or proteins of interest. The method comprising: 1. Simultaneously or sequentially transforming a cell with at least two different expression cassettes in which the gene of interest is linked via an IRES to a fluorescent marker gene. Each marker gene is different. 2. Providing conditions in which expression of the genes will occur. 3. Identifying cells expressing proteins by detecting the different fluorescent signals.

Download PDF
Document Preview
Document History
  • Publication: May 31, 2001
  • Application: Nov 24, 2000
    WO AU 1936/000014 W
  • Priority: Nov 24, 1999
    AU AU PQ422399 A

Download Citation

Sign in to the Lens