A Method For Simultaneous Detection Of Different Types Of Antibodies And/or Antigens Produced By Individual Cells

Abstract

A method for detection of antibodies and/or antigens secreted and/or released by individual cells, at which a cell suspension is brought in contact with a solid carrier. After that or simultaneously therewith are added at least two different types of other antibodies directed against and having an ability to bind to the antibodies or antigens resp. to be detected, and which other antibodies are provided with different enzymes. The corresponding number of indicator substances is then added having different staining, effect for simultaneous detection of the different types of antibodies and/or antigens by evaluation of spots of distinct colours remaining on the solid carrier. According to the embodiment two solid carriers are used, at which antigens and/or antibodies of the same or different type are bound to opposed surfaces of the carriers and the cell suspension is applied between these surfaces.


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Document History
  • Publication: Apr 19, 1990
  • Application: Oct 13, 1989
    WO SE 8900565 W
  • Priority: Jun 16, 1989
    US US 36737789 A
  • Priority: Oct 14, 1988
    SE SE 8803689 A

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