{"search_session":{},"preferences":{"l":"fr","queryLanguage":"fr"},"patentId":"US_9809798_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"049-375-830-867-068"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11626,"type":"PATENT","title":"National University of Singapore - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":4868,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8301,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: nat* univ* AND sing*
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Select all patents in the collection and expand by simple families. Add to collection. Total patents: 4132
Search Applicants and Owners separately: nat* univ* AND sing*
Select more for logical variants. Add to collection.
Select all patents in the collection and expand by simple families. Add to collection. Total patents: 4132
a) culturing blood cells with a matrix to which cells can attach, and one or more carbohydrate-based hydrophilic macromolecules, each of the one or more of the macromolecules has a molecular weight from about 50 kDa to about 1000 kDa, and wherein the one or more macromolecules are present at a concentration from about 2.5 mg/ml to about 100 mg/ml, thereby producing a cell culture; and\n
b) maintaining the cell culture under conditions in which pericyte progenitor cells are generated in the cell culture, wherein the pericyte progenitor cells express platelet-derived growth factor receptor-β (PDGFR-β) and neuron-glial antigen 2 (NG2),\n
c) thereby obtaining pericyte progenitor cells."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 wherein the blood cells are peripheral blood mononuclear cells (PBMCs)."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2 wherein about 100,000 pericyte progenitor cell per 1 ml of blood is obtained."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 wherein the matrix comprises all or a portion of an extracellular matrix."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4 wherein the matrix comprises fibronectin."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 wherein the culture containing the carbohydrate-based hydrophilic macromolecule has a viscosity of less than about 2 mPas."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 wherein the carbohydrate-based hydrophilic macromolecule has a radius of about 2 to about 50 nm."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 wherein the carbohydrate-based hydrophilic macromolecule is a polymer of glucose, sucrose or a combination thereof."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 8 wherein the polymer is Ficoll™70, Ficoll™400, polyvinyl pyrrolidone (PVP), dextran, dextran sulfate, polystyrene sulfonate, pullulan, chondroitan sulfate, heparin, heparan sulfate, dermatan sulfate or a combination thereof."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 wherein the blood cells are contacted with a mixture of carbohydrate-based hydrophilic macromolecules comprising Ficoll™70 and Ficoll™400."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 10 wherein the Ficoll™70 is at a concentration range of from about 7.5 mg/ml to about 100 mg/ml, and the Ficoll™400 is at a concentration range of from about 2.5 mg/ml to about 100 mg/ml."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 11 wherein the Ficoll™70 is at a concentration of about 37.5 mg/ml and the Ficoll™400 is at a concentration of about 25 mg/ml."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 further comprising detecting pericyte progenitor cells in the cell culture."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 13 wherein the detection further comprises detecting cells which adhere to the matrix and express platelet-derived growth factor receptor β (PDGFR-β), neuron-glial antigen 2 (NG2), Tie-2, vascular endothelial growth factor receptor-1 (VEGFR-1), VEGFR-2, CD206 or a combination thereof."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 13 wherein the detection further comprises detecting cells which do not express human leukocyte antigen-DR (HLA-DR), collagen I or a combination thereof."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 wherein the blood cells are contacted with the matrix and one or more carbohydrate-based hydrophilic macromolecules at a seeding concentration of about 10,000 cells/cm2 to about 1,000,000 cells/cm2."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 wherein the conditions under which the cell culture is maintained comprise maintaining the cell culture at about 37° C. in an atmosphere of about 5% CO2/95% air."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 17 wherein the conditions are maintained for about 1 day to about 14 days."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 wherein the blood cells are human cells."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 further comprising isolating the pericyte progenitor cells from the cell culture."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 20 wherein the pericyte progenitor cells are isolated by harvesting cells which adhere to the matrix and express platelet-derived growth factor receptor β (PDGFRβ), neuron-glial antigen 2 (NG2), Tie-2, vascular endothelial growth factor receptor-1 (VEGFR-1), VEGFR-2, CD206 or a combination thereof."],"number":21,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}