Methods For Phenotyping Of Intact Whole Tissues

Abstract

In various embodiments, the present application teaches methods and compositions for tissue clearing in which whole organs and bodies are rendered macromolecule-permeable and optically-transparent, thereby exposing their cellular structure with intact connectivity. In some embodiments, the present application teaches PACT, a protocol for passive tissue clearing and immunostaining of intact organs. In other embodiments, the present application teaches RIMS, a refractive index matching media for imaging thick tissue. In yet other embodiments, the application teaches PARS, a method for whole-body clearing and immunolabeling.


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Document History
  • Publication: Oct 3, 2017
  • Application: Jul 30, 2014
    US US 201414447607 A
  • Priority: Jul 30, 2014
    US US 201414447607 A
  • Priority: May 12, 2014
    US US 201461992103 P
  • Priority: Sep 20, 2013
    US US 201361880401 P

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