{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_9725698_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"132-260-182-612-996"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11993,"type":"PATENT","title":"University of Connecticut Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":3197,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8558,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:Univ* Connecti*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2880
Search Applicants and Owners separately:Univ* Connecti*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2880
(a) culturing hESCs or iPSCs in a medium comprising bone morphogenetic protein-4 (BMP-4), and optionally a TGFβ inhibitor, for a first time period of 1 to 5 days sufficient for the hESCs or iPSCs to differentiate into trophoblast cells;\n
(b) dissociating the trophoblast cells into single trophoblast cells; and\n
(c) plating the single trophoblast cells from step (b) onto gelatin, vitronectin, laminin, fibronectin, Matrigel or collagen-coated plates, and culturing said single trophoblast cells for a second time period of 4 to 10 days in a mesenchymal stem cell (MSC) growth medium containing LIF, bFGF or PDGF, or a combination thereof, thereby producing human T-MSCs."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, where the TGFβ inhibitor is an SB431542, A83-01 or ALK5 inhibitor."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 wherein, prior to step (a), hESCs are cultured by a method comprising the following steps:\n
(i) culturing the hESCs to about 80% confluency on Matrigel-coated plates;\n
(ii) dissociating the hESCs under suitable conditions;\n
(iii) isolating the hESCs; and\n
(iv) washing the hESCs."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the concentration of BMP4 is about 1 to about 100 ng/ml."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the population of human T-MSCs: (i) comprises greater than 95% of cells expressing CD73, CD90, CD105, CD146, CD166, and CD44; (ii) comprises greater than 80% of cells expressing CD13, CD29, CD54, CD49E; (iii) comprises less than 5% of cells expressing CD45, CD34, CD31 and SSEA4; (iv) expresses IL-10 and TGFβ; (v) comprises less than 2% of cells expressing IL-6, IL-12 and TNFα; and (vi) comprises less than 0.001% of cells co-expressing OCT4, NANOG, TRA-1-60 and SSEA4."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 5, wherein the human T-MSCs do not express MMP2 and RAGE."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 5, wherein the T-MSC cells have low expression of IFNγR1 and IFNγR2 compared to expression of IFNγR1 and IFNγR2 of bone marrow-derived mesenchymal stem cells (BM-MSC)."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 5, wherein the human T-MSCs further express CD73 and do not express IL-6."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 5, wherein the human T-MSCs further express at least one cell marker selected from the group consisting of CD90, CD105, CD13, CD29, CD54, CD146, CD166, and CD44; do not express at least one marker selected from the group consisting of CD34, CD31, and CD45; and do not express at least one marker selected from the group consisting of MMP, RAGE, IFNγR1, IFNγR2, IL-12, TNFα and VCAM1."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 5, wherein the human T-MSCs are irradiated."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 7, wherein the human T-MSCs are irradiated with gamma-irradiation."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["A method of producing human trophoblast-derived mesenchymal stem cells from human embryonic stem cells (hESCs) or induced pluripotent stem cells (iPSCs), the method comprising:\n
(a) culturing hESCs or iPSCs in a medium comprising bone morphogenetic protein-4 (BMP-4) for 1½ to 5 days for the hESC or iPSC to differentiate into trophoblast cells, and optionally with a TGFβ inhibitor;\n
(b) isolating the trophoblast cells;\n
(c) dissociating the trophoblast cells from step (b) into single trophoblast cells; and\n
(d) plating the single trophoblast cells from step (c) onto Matrigel-coated plates and culturing said single trophoblast cells for 4-10 days in a mesenchymal stem cell (MSC) growth medium containing serum, knockout serum replacement (KOSR), or in a serum-free medium, in an amount sufficient to induce differentiation of the single trophoblast cells into human trophoblast-derived mesenchymal stem cells; wherein at least 90% of the human trophoblast-derived mesenchymal stem cells express CD73."],"number":12,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}