{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_9506915_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"010-031-943-833-185","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":12138,"type":"PATENT","title":"University of Arkansas at Fayetteville - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":3026,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8643,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:Univ* Arkansas. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2874
Search Applicants and Owners separately:Univ* Arkansas. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2874
a) providing:\n\ni) a first cell sample comprising nucleic acid binding proteins and the target chromatin, wherein the target chromatin is tagged by contacting the target chromatin with a tag capable of specifically recognizing and binding one or more portions of the target chromatin and wherein the tag comprises a nucleic acid engineered to have binding specificity for a nucleic acid sequence component of the target chromatin, a protein that associates with the nucleic acid engineered to have binding specificity for a nucleic acid sequence component of the target chromatin, and an affinity handle, and\nii) a second cell sample comprising nucleic acid binding proteins and the target chromatin, wherein the target chromatin is not tagged by contacting the target chromatin with a non-functional tag that is not capable of specifically recognizing and binding one or more portions of the target chromatin and wherein the non-functional tag comprises a protein capable of associating with a nucleic acid engineered to have binding specificity for a nucleic acid sequence component of the target chromatin and an affinity handle, wherein the non-functional tag does not comprise a nucleic acid engineered to have binding specificity for a nucleic acid sequence component of the tartget chromatin,\nwherein the first cell sample and the second cell sample are lysed;\n
b) performing affinity purification on each lysed cell sample in (a) using a substrate capable of binding the affinity handle, wherein affinity purification of the first cell sample results in isolation of affinity handle bound to tagged target chromatin bound to nucleic acid binding proteins and affinity purification of the second cell sample results in isolation of nucleic acid binding proteins non-specifically bound to the substrate, wherein isolating the affinity handle isolates proteins associated with the tagged target chromatin;\n
c) identifying bound proteins from (b); and\n
d) determining the amount of each bound protein in each cell sample from (b), wherein bound proteins that are enriched in the first cell sample as compared to the second cell sample are specifically associated with the tagged chromatin in the first cell sample."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the nucleic acid engineered to have binding specificity for a nucleic acid sequence component of the target chromatin is a guide RNA (g RNA)."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2, wherein the protein that associates with the gRNA is a nuclease inactivated Cas9 protein, or derivative thereof."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the target chromatin in the first cell sample is tagged by expressing in a cell a tag comprising a nucleic acid engineered to have binding specificity for a nucleic acid sequence component of the target chromatin."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 4, wherein the nucleic acid engineered to have binding specificity for a nucleic acid sequence component of the target chromatin is a guide RNA."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the target chromatin is tagged by expressing in a cell a gRNA engineered to have binding specificity for a nucleic acid sequence component of the target chromatin, a nuclease inactivated Cas9 protein that associates with the gRNA, and an affinity handle."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the second cell sample is contacted with a non-functional tag by expressing in a cell a protein capable of associating with a nucleic acid engineered to have binding specificity for a nucleic acid sequence component of the target chromatin and an affinity handle, wherein the nucleic add engineered to have binding specificity for a nucleic acid sequence component of the target chromatin is not expressed in the cell."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the chromatin is fragmented to comprise nucleic acid sections comprising 500 to 1500 base pairs."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the target chromatin in the first cell sample is contacted with a tag during cell culture and the target chromatin in the second cell sample is contacted with a non-functional tag during cell culture."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the target chromain in the first cell sample is contacted with a tag following cell lysis and the target chromatin in the second cell sample is contacted with a non-functional tag following cell lysis."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the first and second cell samples are biological samples."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the first cell sample and the second cell sample are crosslinked and then lysed."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the identifying of step (c) involves mass spectrometry."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein step (d) involves label-free proteomics."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14, wherein the label-free proteomics technique is spectral counting."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein proteins enriched in the first cell sample compared to the second cell sample are enriched by at least 2 fold."],"number":16,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}