{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_9220810_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"116-893-834-407-219"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11766,"type":"PATENT","title":"Florida State University Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":2590,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8404,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: florida state uni*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2443
Search Applicants and Owners separately: florida state uni*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2443
a) seeding freshly isolated mammalian MSC on a planar surface or a porous three-dimension (3-D) scaffold, wherein said surface or scaffold comprises one or more thermally responsive films or coatings;\n
b) growing said mammalian MSC under an O2 tension that is lower than 20%;\n
c) decellularizing said surface or scaffold; and\n
d) reseeding said surface or scaffold with freshly isolated mammalian MSC, whereby said reseeded mammalian MSC grow and maintain an undifferentiated phenotype."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said O2 tension is between about 1% and 10%; or about 1% and 5%; or about 1% and 3%; or is about 2%."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said decellularizing step is performed using a detergent and an enzyme that degrades nucleic acid."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, further comprising the step of harvesting said reseeded MSC from said surface or scaffold."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said mammalian MSC are human MSC."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said mammalian MSC are isolated from bone marrow or adipose tissue of a mammal."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said surface or scaffold is non-degradable."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said surface or scaffold is composed of one or more of poly(styrene), poly(carprolactone), nylon, or poly(ethylene terephthalate) (PET)."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said reseeded cells are allowed to grow on said surface or scaffold for up to about three months."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said thermally responsive film or coating comprises one or more of N-isopropylacrylamide, poly(allylamine hydrochloride)-co-poly(N-isopropylacrylamide), or poly(styrene sulfonate)-co-poly(N-isopropylacrylamide)."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said one or more thermally responsive films or coatings comprises a terminal layer of positively charged allylamine hydrochloride (PAH), or negatively charged styrene sulfonic acid (PSS), or serum."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said reseeded MSC are harvested from said surface by modulating the temperature of said thermally responsive film or coating to a temperature where cells adhered thereto are released."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 12, wherein said harvested MSC are further grown and cultivated in suspension culture."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said MSC are grown on said scaffold in a perfusion bioreactor."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said MSC are grown in cell culture media comprising an animal serum at a concentration less than about 5% v/v or in serum-free cell culture media."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["A composition comprising a decellularized extracellular matrix (ECM) prepared in vitro by:\n
a) seeding freshly isolated mammalian MSC on a planar surface or a porous three-dimension (3-D) scaffold, wherein said surface or scaffold comprises one or more thermally responsive films or coatings;\n
b) growing said mammalian MSC under an O2 tension that is lower than 20%; and\n
c) decellularizing said surface or scaffold."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The composition of claim 16, wherein said O2 tension is between about 1% and 10%; or about 1% and 5%; or about 1% and 3%; or is about 2%."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The composition of claim 16, wherein said decellularizing step is performed using a detergent and an enzyme that degrades nucleic acid."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["The composition of claim 16, wherein said surface or scaffold is composed of one or more of poly(styrene), poly(carprolactone), nylon, or poly(ethylene terephthalate) (PET)."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["The composition of claim 16, wherein said thermally responsive film or coating comprises one or more of N-isopropylacrylamide, poly(allylamine hydrochloride)-co-poly(N-isopropylacrylamide), or poly(styrene sulfonate)-co-poly(N-isopropylacrylamide)."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["The composition of claim 16, wherein said one or more thermally responsive films or coatings comprises a terminal layer of positively charged allylamine hydrochloride (PAH), or negatively charged styrene sulfonic acid (PSS), or serum."],"number":21,"annotation":false,"title":false,"claim":true},{"lines":["A composition comprising a decellularized extracellular matrix (ECM) prepared in vitro by:\n
a) seeding freshly isolated mammalian MSC on a planar surface or a porous three-dimension (3-D) scaffold, wherein said surface or scaffold is composed of one or more of poly(styrene), poly(carprolactone), nylon, or poly(ethylene terephthalate) (PET);\n
b) growing said mammalian MSC under an O2 tension that is lower than 20%; and\n
c) decellularizing said surface or scaffold."],"number":22,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}