High Affinity Recombinant Sea Lamprey Antibodies Selected By A Yeast Surface Display Platform

Abstract

The present invention relates to a Yeast Surface Display (YSD) vector for expression of VLR proteins by yeast, wherein the vector includes nucleotide sequences encoding segments of yeast flocculation proteins Flo1p, such as the leader and C-terminal segments, a homologous recombinant cassette and a geneticin/kanamycin resistance gene. The vector can be used for expression of VLR that may be effective in diagnostic applications (e.g., protein chip, immunohistochemistry, flow cytometry), immunoaffinity purification, and for engineering novel fusion proteins.


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Document History
  • Publication: Sep 8, 2015
  • Application: Nov 25, 2009
    US US 200913132086 A
  • Priority: Nov 25, 2009
    US US 200913132086 A
  • Priority: Nov 25, 2009
    US US 2009/0065852 W
  • Priority: Dec 1, 2008
    US US 11892208 P

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