{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_8900572_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"190-612-928-161-651"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11780,"type":"PATENT","title":"Laval University Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":3504,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8414,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: Laval Univ*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1977
Search Applicants and Owners separately: Newcastle Univ*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1333
Search Applicants and Owners separately: Laval Univ*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1977
Search Applicants and Owners separately: Newcastle Univ*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1333
i) Treating said iPSCs with at least one myogenic factor protein;\n
ii) Inducing said at least one myogenic factor expression in said iPSCs; or\n
iii) Introducing in said iPSCs at least one nucleic acid capable of expressing said at least one myogenic factor."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 7, wherein said at least one myogenic factor is MyoD, Pax3, Pax7, Myf5 or myogenin."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 8, wherein said at least one myogenic factor is MyoD."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 9, wherein said MyoD is provided by introducing in said iPSCs a nucleic acid sequence capable of expressing MyoD."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 10, wherein said MyoD is introduced in said iPSCs with an adeno-associated viral vector; a retroviral vector, a lentiviral vector, a non-integrative lentiviral vector or a non-viral vector."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 11, comprising growing said iPSCs as single cells."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 12, wherein said myogenic cells express at least one of the following myogenic markers: Pax7, MyoD, myogenin, CD56, desmin and MHC and express low levels of Rex-1."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2, wherein said myogenic differentiation medium comprises between about 0.5 and about 2% of serum."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2, wherein said myogenic differentiation medium comprises between about 0.5% and about 5% of serum."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said iPSCs are derived from a fibroblast of a Duchenne muscular dystrophy patient."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["A method of transplanting myogenic cells in a subject comprising implanting in said subject myogenic cells prepared by i) culturing human induced pluripotent stem cells (iPSCs) in a myogenic differentiation medium to induce differentiation into mesenchymal-like cells expressing TBX1 and TBX4; and ii) culturing said mesenchymal-like cells in the presence of a myogenic factor to complete differentiation into myogenic cells."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 17, wherein said transplantation is for increasing muscle mass in a subject suffering from Duchenne muscular dystrophy."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 17, wherein said myogenic differentiation medium is substantially the same as Myogenic Culture Medium 1 (MCM1)."],"number":19,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}