{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_8741603_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"182-112-001-932-632","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11855,"type":"PATENT","title":"University of Montpellier Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":11808,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8467,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:univ* AND Montpellier. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1289
Search Applicants and Owners separately:univ* AND Montpellier. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1289
integrating a polynucleotide encoding an oxygen binding protein into a base S. spinosa strain to produce a modified S. spinosa strain; and\n
expressing the polynucleotide in the modified S. spinosa strain;\n
wherein the titer of a spinosyn is increased by at least 4.6% in the modified S. spinosa strain relative to the titer of the spinosyn in the base S. spinosa strain as may be determined by comparing the spinosyn titer of the base S. spinosa strain to the spinosyn titer of the modified S. spinosa strain after 10 days of fermentation under conditions appropriate for the production of spinosyn."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the oxygen binding protein is a globin protein."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2 wherein the globin protein is selected from the group consisting of Vitreoscilla hemoglobin, Alcaligenes eutrophus flavohemoprotein, horse heart myoglobin, Escherichia coli hemoprotein, Bacillus subtilis hemoprotein, yeast flavohemoglobin, soybean leghemoglobin, lupin leghemoglobin, and sperm whale myoglobin."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2 wherein the globin protein is Vitreoscilla hemoglobin or a functional polymorph thereof that increases spinosyn titers."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the oxygen binding protein is integrated into chromosomal DNA in a neutral site in a S. spinosa genome."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the spinosyn titers that are increased are selected from the group consisting of spinosyn A+D, spinosyn J+L, spinosyn A and spinosyn J."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["A method of producing spinosyn production the method comprising:\n
culturing a Saccharopolyspora spinosa that expresses a heterologous gene encoding an oxygen-binding protein under conditions appropriate for production of spinosyn; and collecting spinosyn from a culture of a spinosyn producing organism that expresses a heterologous gene encoding the oxygen-binding protein."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 7 wherein the heterologous gene is integrated into a chromosome of the S. spinosa. "],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["A method for integrating a polynucleotide encoding an oxygen binding protein into chromosomal DNA of a strain of Saccharopolyspora spinosa species comprising the following steps:\n
creating a phage library to identify a genomic locus;\n
cloning two genomic DNA fragments from the genomic locus, wherein one genomic fragment is cloned upstream of the polynucleotide encoding an oxygen binding protein, and the second genomic fragment is cloned downstream of said polynucleotide;\n
introducing the genomic fragments into a strain of S. spinosa by transformation;\n
obtaining transconjugants; and,\n
screening said transconjugants for presence of said polynucleotide."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9 wherein the polynucleotide contains a gene expression cassette."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The gene expression cassette of claim 10, wherein the gene expression cassette contains an antibiotic selectable marker."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The gene expression cassette of claim 10, wherein the gene expression cassette contains a spinosyn enhancing gene expression cassette."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9 wherein the oxygen binding protein is a globin protein."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 13 wherein the globin protein is selected from the group consisting of Vitreoscilla hemoglobin, Alcaligenes eutrophus flavohemoprotein, horse heart myoglobin, E. coli hemoprotein, B. subtilis hemoprotein, yeast flavohemoglobin, soybean leghemoglobin, lupin leghemoglobin, and sperm whale myoglobin."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9 wherein the chromosomal DNA selected is in a neutral site in a S. spinosa genome."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9 wherein the chromosomal DNA selected is an obscuring polyketide synthase cluster."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["An recombinant S. spinosa organism, wherein the organism further expresses a heterologous oxygen binding protein."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The organism of claim 17, wherein the oxygen-binding protein is a globin protein."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The organism of claim 18, wherein the globin protein is selected from the group consisting of Vitreoscilla hemoglobin, Alcaligenes eutrophus flavohemoprotein, horse heart myoglobin, E. coli hemoprotein, B. subtilis hemoprotein, yeast flavohemoglobin, soybean leghemoglobin, lupin leghemoglobin, and sperm whale myoglobin."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["The organism of claim 17, wherein the spinosyn is selected from the group consisting of spinsoyn A+D, spinosyn J+L, spinosyn A and spinosyn J."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The organism of claim 18 wherein the globin protein is Vitreoscilla hemoglobin or a functional polymorph thereof that increases spinosyn titers."],"number":21,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}