Abstract
The present invention relates to a method for modulating the expression of epidermal growth factor receptor (EGFR) and its downstream signalling effectors. In particular, the present invention relates to a method for modulating the expression of epidermal growth factor receptor (EGFR) or like molecule in a cell comprising contacting said cell with an agent capable of directly or indirectly interacting with the 3'-untranslated region (UTR) of the mRNA of said EGFR or like molecule, wherein the UTR is encoded by a sequence which comprises a sequence having at least about 70% identity with at least one sequence selected from the group consisting of the sequences set forth in SEQ ID NOs: 1-36.
Claims
-
A method for reducing the expression of epidermal growth factor receptor (EGFR) or a downstream effector molecule in a cell, comprising:
a) selecting an miRNA capable of interacting with the sequence UCUUCC in the 3′-untranslated region (UTR) of the mRNA of said EGFR or of a downstream effector molecule selected from the group consisting of Raf-1, CALM3, CAMK2D, PAK1, and ADCY9; and
b) contacting said cell with said miRNA under conditions whereby expression of EGFR in said cell is reduced.
- The method of claim 1, wherein the 3′-UTR comprises a sequence which is encoded by a sequence having at least about 90% identity with at least one sequence selected from the group consisting of the sequences set forth in SEQ ID NOs:1-11.
- The method of claim 1, wherein the 3′-UTR is encoded by a sequence contained within the sequence set forth in SEQ ID NO:1.
- The method of claim 1, wherein said contacting comprises contacting said cell with an agent comprising a nucleic acid molecule encoding an miRNA precursor, wherein said precursor is processed in said cell to form said miRNA.
- The method of claim 1, wherein the said miRNA is miR-7.
- The method of claim 5, wherein the miR-7 is the sequence set forth in SEQ ID NO: 48.
- The method of claim 1, wherein the miRNA comprises the sequence set fourth in SEQ ID NO: 52.
- The method of claim 1, wherein the miRNA comprises a nucleic acid molecule having at least about 90% identity with the sequence set forth in SEQ ID NO: 48.
-
A method of inhibiting the growth or differentiation of a cell expressing EGFR or a downstream effector molecule, comprising:
a) selecting an miRNA capable of interacting with the sequence UCUUCC in the 3′-untranslated region (UTR) of the mRNA of said EGFR or of a downstream effector molecule selected from the group consisting of Raf-1, CALM3, CAMK2D, PAK1, and ADCY9; and
b) contacting said cell with said miRNA under conditions whereby growth or differentiation of said cell is inhibited.
- The method of claim 9, wherein-said cell is a cancer cell.
- The method of claim 10, wherein said cancer cell is selected from the group consisting of a brain cancer cell, a lung cancer cell, a breast cancer cell, a prostate cancer cell, and a colon cancer cell.
- The method of claim 10, wherein said cancer cell is from a cancer cell line.
- The method of claim 9, wherein the miRNA comprises the sequence set forth in SEQ ID NO: 52.
- The method of claim 9, wherein the miRNA is miR-7.
- The method of claim 9, wherein the miR-7 is the sequence set forth in SEQ ID NO: 48.
- The method of claim 9, wherein the miRNA comprises a nucleic acid molecule having at least about 90% identity with the sequence set forth in SEQ ID NO: 48.
Owners (US)
-
The University Of Western Australia
(Aug 21 2009)
Explore more patents:
Applicants
-
Leedman Peter
Explore more patents:
-
Giles Keith
Explore more patents:
-
Webster Rebecca Jane
Explore more patents:
-
Univ Western Australia
Explore more patents:
Inventors
CPC Classifications
-
A61K31/7105
Explore more patents:
-
A61K31/711
Explore more patents:
-
C12N15/1135
Explore more patents:
-
C12N15/1138
Explore more patents:
-
C12N2310/14
Explore more patents:
IPC Classifications
-
C12N15/11
Explore more patents:
US Classifications
-
514 44A
Explore more patents:
Download PDF
Document Preview
Document History
- Publication: Mar 18, 2014
-
Application:
Aug 28, 2007
US 31050907 A
-
Priority:
Aug 28, 2007
AU 2007/001247 W
-
Priority:
Feb 6, 2007
AU 2007/900558 A
-
Priority:
Aug 28, 2006
AU 2006/904662 A