{"search_session":{},"preferences":{"l":"ru","queryLanguage":"ru"},"patentId":"US_8524493_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"029-547-654-321-615"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11717,"type":"PATENT","title":"University of Hamburg - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":681,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8370,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: uni* hamburg
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Add to collection. Total patents: 548
Search Applicants and Owners separately:univ* AND Montpellier. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1289
Search Applicants and Owners separately: uni* hamburg
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Add to collection.
Select all patents in the collection and expand by simple families.
Add to collection. Total patents: 548
Search Applicants and Owners separately:univ* AND Montpellier. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1289
isolating a sample from a patient, the sample comprising viable cells;\n
culturing the cells in a cell culture media for a time sufficient for an epithelial cell-specific marker to be released from the viable cells into the cell culture media, the released marker comprising a substantially full-length cytokeratin; and\n
detecting the released epithelial cell-specific marker comprising the substantially full-length cytokeratin that has been released into the cell culture media;\n
wherein detection of the released epithelial cell-specific marker comprising the substantially full-length cytokeratin in the cell culture media indicates the presence of viable disseminated epithelial cells in the sample."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the sample is isolated from blood, bone marrow or lymph nodes."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the sample is depleted of CD-45+ cells."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the released marker comprises a substantially full-length cytokeratin 19."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein the marker comprises a sequence that is at least 90% identical to a full-length polypeptide of SEQ. ID. NO: 1."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein the marker comprises a sequence that is at least 95% identical to a full-length polypeptide of SEQ. ID. NO: 1."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein detecting the released marker comprises binding the released marker with a binding partner."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 7, wherein the binding partner comprises an antibody."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 8, wherein the antibody recognizes an epitope on the marker 5′ to an enzymatic cleavage site on the marker."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein detecting the released marker comprises using mass spectrometry."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 10, wherein the released marker detected by mass spectrometry comprises a polypeptide sequence substantially identical to SEQ. ID. NO: 2, SEQ. ID. NO: 8, SEQ. ID. NO:9, SEQ. ID. NO: 10 or SEQ. ID. NO: 15."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["A method of identifying a substantially full-length epithelial cell marker released from viable cells, the method comprising:\n
isolating a sample from a patient, the sample comprising viable cells;\n
culturing the cells in a cell culture media for a time sufficient for the epithelial cell marker to be released from the viable cells into the cell culture media, the released marker comprising a substantially full-length polypeptide having an enzymatic cleavage site within the full-length polypeptide, the full-length polypeptide comprising substantially full-length cytokeratin; and\n
detecting a portion of the full-length polypeptide comprising the substantially full-length cytokeratin that has been released in the cell culture media, the portion being 5′ to the enzymatic cleavage site;\n
wherein detection of the 5′ portion identifies the full-length epithelial cell marker released from viable cells."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 12, wherein the 5′ portion is detected using an antibody."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 12, wherein the 5′ portion is detected using mass spectrometry."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 14, wherein the 5′ portion comprises a polypeptide sequence substantially identical to SEQ. ID. NO: 2, SEQ. ID. NO: 8, SEQ. ID. NO: 9, SEQ. ID. NO: 10 or SEQ. ID. NO: 15."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 12 wherein the substantially full-length polypeptide comprises cytokeratin 19."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 12, wherein identifying the full-length marker in a sample not normally associated with epithelial cells indicates the presence of a disseminated epithelial tumor cell."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["A method of detecting viable epithelial cells in tissue sample, the method comprising:\n
obtaining a tissue sample from a patient, the sample comprising viable cells;\n
culturing the cells in a cell culture media for a time sufficient for an epithelial cell-specific marker to be released from the viable cells into the cell culture media, the released marker comprising a substantially full-length cytokeratin;\n
detecting the released epithelial cell-specific marker comprising the substantially full-length cytokeratin that has been released into the cell culture media; and\n
wherein detection of the released epithelial cell-specific marker comprising the substantially full-length cytokeratin in the cell culture media indicates the presence of a viable epithelial cell in the tissue sample."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 18, wherein the marker comprises a substantially full-length cytokeratin 19."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 18, wherein the tissue sample is breast tissue."],"number":20,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}