{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_8202717_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"119-010-454-645-810","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":false},"tags":[],"collections":[{"id":8838,"type":"PATENT","title":"Univ of Illinois Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":10245,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8209,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately = \"Univ Illinois\", \"Illinois Univ\", \"University of Illinois\", \"Illinois University\", \"University Illinois NOT Northern NOT southern NOT State\".
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Total patents: 9630
Search Applicants and Owners separately = \"Univ Illinois\", \"Illinois Univ\", \"University of Illinois\", \"Illinois University\", \"University Illinois NOT Northern NOT southern NOT State\".
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Total patents: 9630
a) providing an isolated or purified fetal porcine lung alveolar macrophage cell capable of replicating said PRRS virus, wherein said cell is cultured for at least 5 passages;\n
b) exposing said cell to said PRRS virus; and\n
c) allowing the PRRS virus to replicate in the cell;\n
thereby generating progeny of said PRRS virus."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the cell is from ATCC Patent Deposit No. PTA-8764 or derived therefrom."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, further comprising contacting said cell with a growth factor composition."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 3, wherein said growth factor composition comprises macrophage colony stimulating factor (MCSF) or granulocyte-macrophage colony stimulating factor (GMCSF)."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said cell is obtained from a porcine fetal subject from about 30 to about 90 days of gestational age."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["A method of producing a PRRS vaccine, comprising:\n
providing a modified-live virus (MLV) strain of PRRS virus, and growing said MLV strain in an isolated or purified fetal porcine lung alveolar macrophage cell capable of replicating said PRRSV MLV strain."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, wherein said cell is a primary cell."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, wherein the cell is from ATCC Patent Deposit No. PTA-8764 or derived therefrom."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, further comprising contacting said cell with a growth factor composition."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9, wherein said growth factor composition comprises macrophage colony stimulating factor (MCSF) or granulocyte-macrophage colony stimulating factor (GMCSF)."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, wherein said cell is obtained from a porcine fetal subject from about 30 to about 90 days of gestational age."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["A method of growing a PRRS virus, comprising:\n
(a) isolating a fetal porcine lung alveolar macrophage cell from a porcine fetal lung by steps comprising providing a porcine fetal subject, obtaining a cell-containing bronchoalveolar lavage sample from said subject, and separating said macrophage cell from said sample;\n
(b) culturing said cell; and\n
(c) contacting said cell with said PRRS virus so as to allow viral replication;\n
thereby growing the virus."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12, wherein said culturing comprises passaging said cell for at least 5 passages and/or growing said cell for at least 10 days of continuous culture."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12, wherein said culturing comprises contacting said cell with a growth factor composition."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14, wherein said growth factor composition comprises macrophage colony stimulating factor (MCSF) or granulocyte-macrophage colony stimulating factor (GMCSF)."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12, wherein said cell is obtained from a porcine fetal subject from about 30 to about 90 days of gestational age."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said cell is cultured for at least 10 passages."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said cell is cultured for at least 20 passages."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said cell is a cell line."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, wherein said cell is a cell line."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12, wherein said culturing comprises passaging said cell for at least 20 passages."],"number":21,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}