{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_7799569_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"139-862-675-455-477","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11757,"type":"PATENT","title":"Colorado State University Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":7894,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8396,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: colorado state uni*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1775
Search Applicants and Owners separately: univ* aberdeen. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1658
Search Applicants and Owners separately: colorado state uni*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1775
Search Applicants and Owners separately: univ* aberdeen. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1658
(b) exposing the stained cells to electromagnetic radiation as the stained cells are passed through an interrogation location of a flow cytometer at a rate, R;\n
(c) determining a fluorescence emission characteristic of the exposed cells;\n
(d) using the determined fluorescence characteristic to classify the exposed cells into two or more sub-populations of cells, one of the sub-populations being an enriched sub-population of the first cell type;\n
(e) determining a coefficient of variation for the fluorescence emission characteristic of the cells of the enriched sub-population to provide an indication of sorting efficiency for the staining conditions; and\n
(f) based upon the value of the coefficient of variation for the fluorescence emission characteristic of the cells, determining whether to modify any staining condition under which cells are to be stained or the rate, R, at which the stained cells are to be passed through the interrogation location of the flow cytometer in order to improve sorting efficiency."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 1, wherein said fraction comprises a first fraction and said set of staining conditions comprises a first set of staining conditions, the process further comprising staining a second fraction of said population of cells under a second set of staining conditions different from the first set and performing steps (b) through (e) with said second fraction of the population of cells."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 2, wherein the second fraction is stained based on the coefficient of variation for the fluorescence emission characteristic of the cells of the first fraction."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 2, wherein the second set of staining conditions differs from the first set of staining conditions by at least one of the following: (1) concentration of the fluorescent dye; (2) the length of a staining period used to stain the cells; and (3) a temperature of the cells during a staining period used to stain the cells."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 2, further comprising staining a third fraction of said population of cells under a third set of staining conditions different from the first and second sets and performing steps (b) through (e) with the third fraction of the population of cells."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 5, further comprising staining a fourth fraction of said population of cells under a fourth set of staining conditions different from the first, second, and third sets and performing steps (b) through (e) with the fourth fraction of the population of cells."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 6, further comprising staining a fifth fraction of said population of cells under a fifth set of staining conditions different from the first, second, third and fourth sets and performing steps (b) through (e) with the fifth fraction of the population of cells."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 2, further comprising selectively varying conditions in the first set of staining conditions based upon said determination of the coefficient of variation for the fluorescence emission characteristic of the cells of the first fraction in order to obtain the second set of staining conditions."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 2, wherein a modified staining condition as determined by performing step (f) on the second fraction is applied to the staining of the remainder of the population of cells."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 1, wherein multiple fractions of cells are stained in step (a), each fraction being separately stained under a unique set of staining conditions, wherein steps (b) through (e) are performed for each separately stained fraction, and wherein step (f) comprises using the respective coefficients of variation to determine the set of staining conditions to be used to separately stain additional cells in said population."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 1, wherein the cells are sperm cells."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 11, wherein the first cell type comprises X chromosome bearing sperm cells."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 1, wherein step (a) comprises staining the cells with Hoechst 33342."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 1, wherein said fluorescence emission characteristic comprises a feature of a fluorescence pulse waveform corresponding to movement of a cell through the interrogation location, and wherein said feature is indicative of at least one of the following: (1) total fluorescence intensity; and (2) peak fluorescence intensity."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 1, further comprising determining before step (f) whether the coefficient of variation from step (e) is equal to or less than a predetermined coefficient of variation and repeating steps (a) through (e) using a different set of staining conditions to stain a different fraction of the cells each time until the coefficient of variation determined in step (e) is equal to or less than the predetermined coefficient of variation, and wherein step (f) comprises determining to use the staining conditions to stain additional cells if the coefficient of variation from step (e) for the respective staining conditions is equal to or less than the predetermined coefficient of variation."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["A process for evaluating a set of conditions for staining a population of cells for sorting, the population comprising a first type and a second type of cell, the process comprising.\n
(a) staining a fraction of the population of cells with a fluorescent dye under a set of staining conditions;\n
(b) exposing the stained cells to electromagnetic radiation as the stained cells are passed through an interrogation location of a flow cytometer at a rate, R;\n
(c) determining a fluorescence emission characteristic of the exposed cells;\n
(d) using the determined fluorescence emission characteristic to classify the exposed cells into two or more sub-populations of cells, one of the subpopulations being an enriched sub-population of the first cell type;\n
(e) determining a coefficient of variation for the fluorescence emission characteristic of the cells of the enriched sub-population to provide an indication of sorting efficiency for the staining conditions;\n
(f) based upon the value of the coefficient of variation for the fluorescence emission characteristic of the cells, determining whether to modify any staining condition under which the fraction of cells are to be stained or the rate, R, at which the stained cells are passed through the interrogation location of the flow cytometer in order to improve sorting efficiency; and\n
(g) applying the modified staining condition to the remainder of the population of cells."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 16, further comprising sorting the remainder of the population of cells in a flow cytometer to obtain an enriched sample population of the first cell type."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 16, wherein said fraction comprises a first fraction and said set of staining conditions comprises a first set of staining conditions, the process further comprising staining a second fraction of said population of cells under a second set of staining conditions different from the first set and performing steps (b) through (e) with said second fraction of the population of cells."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 18, wherein the second fraction is stained based on the coefficient of variation for the fluorescence emission characteristic of the cells of the first fraction."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 18, wherein the second set of staining conditions differs from the first set by at least one of the following: (1) concentration of the fluorescent dye; (2) the length of a staining period used to stain the cells; and (3) the temperature of the cells during a staining period used to stain the cells."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 18, further comprising staining a third fraction of said population of cells under a third set of staining conditions different from the first and second sets and performing steps (b) through (f) with the third fraction of the population of cells."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 21, further comprising staining a fourth fraction of said population of cells under a fourth set of staining conditions different from the first, second, and third sets and performing steps (b) through (f) with the fourth fraction of the population of cells."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 22, further comprising staining a fifth fraction of said population of cells under a fifth set of staining conditions different from the first, second, third and fourth sets and performing steps (b) through (f) with the fifth fraction of the population of cells."],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 18, further comprising prior to step (g), selecting a set of staining conditions for use in step (g) that results in a coefficient of variation level for the fluorescence emission characteristic that is equal to or less than a predetermined coefficient of variation."],"number":24,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 24, wherein the predetermined coefficient of variation is about 1.3% or less."],"number":25,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 16, further comprising determining before step (f) whether the coefficient of variation from step (e) is equal to or less than a predetermined coefficient of variation and repeating steps (a) through (e) using a different set of staining conditions to stain a different fraction of the cells each time until the coefficient of variation determined in step (e) is equal to or less than the predetermined coefficient of variation, and wherein step (f) comprises determining to use the staining conditions to stain additional cells if the coefficient of variation from step (e) for the respective staining conditions is equal to or less than the predetermined coefficient of variation."],"number":26,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 16, wherein the cells are sperm cells."],"number":27,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 27 wherein the first cell type comprises X chromosome bearing sperm cells."],"number":28,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 16, wherein step (a) comprises staining the cells with Hoechst 33342."],"number":29,"annotation":false,"claim":true,"title":false},{"lines":["The process of claim 16, wherein said fluorescence emission characteristic comprises a feature of a fluorescence pulse waveform corresponding to movement of a cell through the interrogation location, and wherein said feature is indicative of at least one of the following: (1) total fluorescence intensity; and (2) peak fluorescence intensity."],"number":30,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}