{"search_session":{},"preferences":{"l":"es","queryLanguage":"es"},"patentId":"US_7785825_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"136-905-509-742-882"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8838,"type":"PATENT","title":"Univ of Illinois Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":10245,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8209,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately = \"Univ Illinois\", \"Illinois Univ\", \"University of Illinois\", \"Illinois University\", \"University Illinois NOT Northern NOT southern NOT State\".
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Total patents: 9630
Search Applicants and Owners separately = \"Univ Illinois\", \"Illinois Univ\", \"University of Illinois\", \"Illinois University\", \"University Illinois NOT Northern NOT southern NOT State\".
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Total patents: 9630
(a) providing said lantibiotic precursor peptide;\n
(b) providing a purified LctM enzyme capable of catalyzing dehydration and cyclization of the lantibiotic precursor peptide, wherein said LctM enzyme has at least 95% sequence identity to SEQ ID NO:40 and enzymatic activity of dehydration or dehydration and cyclization of the lantibiotic precursor peptide of SEQ ID NO:3; and\n
(c) incubating a reaction mixture comprising a divalent metal cation, ATP, said lantibiotic precursor peptide of (a) and said LctM enzyme; thereby modifying said lantibiotic precursor peptide by dehydration or dehydration and cyclization."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the lantibiotic precursor peptide comprises at least one non-proteinogenic amino acid, unnatural amino acid, beta amino acid, peptoid, or derivatized amino acid."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the reaction mixture further comprises magnesium."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the reaction mixture further comprises magnesium and zinc."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the reaction mixture further comprises magnesium, zinc, and DTT."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["A method of generating a biologically active lantibiotic compound, comprising:\n
(a) incubating a reaction mixture comprising a divalent metal ion, ATP, a lantibiotic precursor peptide and a purified LctM enzyme having at least 95% identity to SEQ ID NO:40 and enzymatic activity of dehydration or dehydration and cyclization of the lantibiotic precursor peptide of SEQ ID NO: 3; and\n
(b) cleaving a leader portion of the lantibiotic precursor peptide; thereby generating said biologically active lantibiotic compound."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 6, wherein said divalent metal cation is zinc or magnesium."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["A method of modifying a lantibiotic precursor peptide by dehydration or dehydration and cyclization, comprising:\n
(a) providing said lantibiotic precursor peptide;\n
(b) providing a purified LctM enzyme capable of catalyzing dehydration and cyclization of the lantibiotic precursor peptide, wherein said LctM enzyme comprises the amino acid sequence as set forth in SEQ ID NO:40; and\n
(c) incubating a reaction mixture comprising a divalent metal cation, ATP, said lantibiotic precursor peptide of (a) and said LctM enzyme; thereby modifying said lantibiotic precursor peptide by dehydration or dehydration and cyclization."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 8, wherein said LctM enzyme consists of the sequence set forth in SEQ ID NO:40."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 9 wherein said divalent metal cation is zinc or magnesium."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 9 wherein the reaction mixture further comprises DTT."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 9, wherein the reaction mixture further comprises DTT."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["A method of generating a biologically active lantibiotic compound, comprising:\n
(a) incubating a reaction mixture comprising a divalent metal ion, ATP, a lantibiotic precursor peptide and a purified LctM enzyme comprising the amino acid sequence as set forth in SEQ ID NO: 40; and\n
(b) cleaving a leader portion of the lantibiotic precursor peptide; thereby generating said biologically active lantibiotic compound."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 13, wherein said LctM enzyme consists of the sequence set forth in SEQ ID NO:40."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 13, wherein the lantibiotic precursor peptide comprises at least one non-proteinogenic amino acid, unnatural amino acid, beta amino acid, peptoid, or derivatized amino acid."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 13, wherein said divalent metal cation is zinc or magnesium."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 13, wherein said reaction mixture further comprises DTT."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 9, wherein the lantibiotic precursor peptide comprises at least one non-proteinogenic amino acid, unnatural amino acid, beta amino acid, peptoid, or derivatized amino acid."],"number":18,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}