{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_7758854_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"100-022-645-093-447","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8892,"type":"PATENT","title":"University of Wisconsin Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":15082,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8214,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners = \"Wisconsin University\" , \"University of Wisconsin\", \"Wisconsin Univ\", \" Univ Wisconsin\" , \"Wisco* Alum* Res* Found*\".
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Search Applicants and Owners = \"Wisconsin University\" , \"University of Wisconsin\", \"Wisconsin Univ\", \" Univ Wisconsin\" , \"Wisco* Alum* Res* Found*\".
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administering to the subject donor bacterial cells harboring a transmissible plasmid that comprises\n
a) an origin of replication for synthesizing the plasmid in a bacterial cell, wherein initiation of replication at the origin is negatively controlled by a plasmid replication protein comprising a copy number control function, wherein in the absence of the plasmid replication protein copy number control function, the transmissible plasmid undergoes runaway replication,\n
b) a mutant gene encoding a plasmid replication protein comprising a copy number control function, wherein said mutant gene encoding said plasmid replication protein comprises a mutation that reduces the copy number control function of said plasmid replication protein,\n
c) an origin of transfer from which conjugative transfer of the transmissible plasmid initiates from the donor bacterial cells to target bacterial cells, and\n
d) at least one screenable marker gene, wherein each donor bacterial cell further comprises one or more transfer genes conferring upon the donor bacterial cell the ability to conjugatively transfer the transmissible plasmid to a target bacterial cell, wherein each donor bacterial cell further comprises a wild type gene encoding said plasmid replication protein comprising a copy number control function, wherein the target bacterial cells that do not produce the plasmid replication protein comprising a copy number control function, thereby enabling the transmissible plasmid to undergo runaway replication in the target bacterial cells, and wherein the donor bacterial cells are administered in a manner such that the donor bacterial cells contact the target bacterial cells and conjugatively transfer from the donor bacterial cells to the target bacterial cells, whereupon the transmissible plasmid undergoes runaway replication, thereby killing the target bacterial cells."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the subject is a human or an animal."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the subject is a plant."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the subject is food or feed."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein a donor bacterial cell further comprises a helper plasmid, wherein said one or more transfer genes are contained on said helper plasmid, such that the transmissible plasmid is transmissible from the donor bacterial cell to a target bacterial cell, but is not further transmissible from the target bacterial cell to another target bacterial cell."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said one or more transfer genes are contained on the transmissible plasmid, such that the transmissible plasmid is transmissible from the donor bacterial cell to a target bacterial cell, and further from the target bacterial cell to another target bacterial cell."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said transfer genes are those of a plasmid selected from F, R6K and Ti."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said plasmid replication protein is a bifunctional protein comprising a plasmid replication activator function and a plasmid replication inhibitor function, wherein when the plasmid replication activator function is present and when the plasmid replication inhibitor function is reduced, the transmissible plasmid undergoes runaway replication, and wherein said mutant gene encoding a plasmid replication protein comprising a copy number control function is a mutant gene encoding a bifunctional plasmid replication protein comprising a plasmid replication activator function and a plasmid replication inhibitor function, wherein said gene encoding said bifunctional plasmid replication protein comprises a mutation that reduces the plasmid replication inhibitor function of said bifunctional plasmid replication protein."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 8, wherein said gene encoding said bifunctional protein comprises a pir gene from plasmid R6K, and wherein said pir gene encodes a π protein (SEQ ID NO:2)."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 8, wherein said gene encoding a mutant variant of said bifunctional protein comprises a mutant variant of a pir gene from plasmid R6K, and wherein said mutant variant of said bifunctional protein is a mutant variant of a non-mutant π protein (SEQ ID NO:2)."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 10, wherein said mutant variant of said π protein comprises at least one amino acid deletion or substitution relative to the amino acid sequence of said non-mutant π protein (SEQ ID NO:2)."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 11, wherein said at least one amino acid deletion or substitution comprises a deletion or substitution at amino acid 105, 106 or 107 of the amino acid sequence of said non-mutant π protein (SEQ ID NO:2)."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 11, wherein said at least one amino acid deletion or substitution comprises a deletion or substitution at amino acids 106 and 107 of the amino acid sequence of said non-mutant π protein (SEQ ID NO:2)."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the origin of replication is that of a plasmid selected from R6K, RK2, rts1, p15A and RSF1010."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the origin of replication is selected from F and P1."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the transmissible plasmid comprises a portion of a plasmid selected from RK2, R6K, pCU1, p15A, pIP501, pAMβ1 and pCRG1600."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the donor bacterial cell is selected from dividing bacteria, maxicells, minicells and conditionally non-functional cells."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the donor bacterial cell is selected from Escherichia coli, Lactobacillus spp., Lactococcus, Bifidobacteria, Eubacteria, and bacterial minicells."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the target bacterial cell is selected from the group consisting of Campylobacter spp., Enterobacter spp., Enterococcus spp., Escherichia coli, Gardnerella vaginalis, Haemophilis spp., Helicobacter pylori, Mycobacterium tuberculosis, Propionobacter acnes, Pseudomonas aeruginosa and other Pseudomonas spp., Salmonella typhimurium, Shigella spp. and Staphylococcus spp."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["A method of reducing or eliminating a target population of bacteria in a subject, the method comprising:\n
administering to the subject donor bacterial cells harboring a transmissible plasmid that comprises\n
a) an origin of replication for synthesizing the plasmid in a bacterial cell, wherein initiation of replication at the origin is negatively controlled by a plasmid replication protein comprising a copy number control function, wherein in the absence of the plasmid replication protein copy number control function, the transmissible plasmid undergoes runaway replication,\n
b) a mutant gene encoding a plasmid replication protein comprising a copy number control function, wherein said mutant gene encoding said plasmid replication protein comprises a mutation that reduces the copy number control function of said plasmid replication protein,\n
c) an origin of transfer from which conjugative transfer of the transmissible plasmid initiates from the donor bacterial cells to target bacterial cells, and\n
d) at least one screenable marker gene;\n
wherein the transmissible plasmid is selected from the group consisting of R6K, RK2, pCU1, P15A, pIP501, pAMβ1 and pCRG1600;\n
wherein each donor bacterial cell further comprises:\n\ni) one or more transfer genes conferring upon the donor bacterial cell the ability to conjugatively transfer the transmissible plasmid to a target bacterial cell, and\nii) a wild type gene encoding said plasmid replication protein comprising a copy number control function,\n
wherein the target bacterial cells do not produce the plasmid replication protein comprising a copy number control function, thereby enabling the transmissible plasmid to undergo runaway replication in the target bacterial cells, and\n
wherein the donor bacterial cells are administered in a manner such that the donor bacterial cells contact the target bacterial cells and conjugatively transfer from the donor bacterial cells to the target bacterial cells, whereupon the transmissible plasmid undergoes runaway replication, thereby killing the target bacterial cells."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 20 wherein the wherein the target bacterial cell is selected from the group consisting of Campylobacter spp., Enterobacter spp., Enterococcus spp., Escherichia coli, Gardnerella vaginalis, Haemophilis spp., Helicobacter pylori, Mycobacterium tuberculosis, Propionobacter acnes, Pseudomonas aeruginosa and other Pseudomonas spp., Salmonella typhimurium, Shigella spp. and Staphylococcus spp."],"number":21,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}