{"search_session":{},"preferences":{"l":"ru","queryLanguage":"ru"},"patentId":"US_7709257_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"142-978-666-269-587"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11749,"type":"PATENT","title":"Virginia Commonwealth University Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":1918,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8388,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: virginia common* uni*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1730
Search Applicants and Owners separately: virginia common* uni*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1730
a) prepulsing a population of dendritic cells (DCs) with a selected antigen to obtain an antigen-prepulsed DC population;\n
b) culturing the antigen-prepulsed DC population of a) with a population of T cells to obtain a T/DC/Ag cell population;\n
c) prepulsing a population of B cells with the selected antigen followed by removal of the antigen to obtain an antigen-prepulsed B cell population (B-Ag); and\n
d) co-culturing the T/DC/Ag cell population of b) with the B-Ag cell population of c), after at least about 24 hours of culturing in b), to obtain a T/DC+B-Ag co-culture under conditions permitting a B cell response; wherein the number of B cells producing antibody in the T/DC+B-Ag co-culture is greater than the number of B cells producing antibody in a co-culture in which the T cells were co-cultured with an antigen-prepulsed DC population for less than 24 hours prior to addition of antigen-prepulsed B cells; thereby enhancing antigen-induced responsiveness of B cells in a population."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the culturing of b) is for about three days."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["An in vitro method of enhancing antigen-induced responsiveness of B cells in a population comprising:\n
a) prepulsing a population of dendritic cells (DCs) with a selected antigen to obtain a first antigen-prepulsed DC population;\n
b) culturing the first antigen-prepulsed DC population of a) with a population of T cells to obtain a T/DC/Ag cell population;\n
c) prepulsing a second population of DCs with the selected antigen to obtain a second antigen-prepulsed DC population;\n
d) culturing the second antigen-prepulsed DC population of c) with a population of B cells to obtain a B cell-dendritic cell population (B-DC);\n
e) co-culturing the T/DC/Ag cell population of b) with the B-DC cell population of d) after at least about 24 hours of culturing in b), to obtain a T/DC+B-DC co-culture under conditions permitting a B cell response; wherein the number of B cells producing antibody in the T/DC+B-DC co-culture is greater than the number of B cells producing antibody in a co-culture in which the T cells were co-cultured with an antigen-prepulsed DC population for less than 24 hours prior to addition of B cell-dendritic cells; thereby enhancing antigen-induced responsiveness of B cells in a population."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, wherein the culturing of b) is for about three days."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, further comprising: e) separating antibody-producing B cells from the T/DC+B-Ag co-culture."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, further comprising: e) separating antibody-producing B cells from the T/DC+B-DC co-culture."],"number":6,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}