Modified Xylanase Exhibiting Improved Expression


A modified Family 11 xylanase enzyme comprising a sequence that introduces a functional consensus glycosylation site is provided. Non-limiting examples of introduced glycosylation sites include mutation of the amino acid at position 34, 131, 180, 182, or a combination thereof, to an asparagine. The indicated amino acid position in the Family 11 xylanase is determined from sequence alignment of the xylanase of interest with that of a Trichoderma reesei xylanase II amino acid sequence. The introduced consensus glycosylation site facilitates increased expression efficiency of the modified xylanase when compared to the expression efficiency of a corresponding xylanase from which the modified xylanase was derived, using similar host strains and growth conditions.

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Document History
  • Publication: Apr 6, 2010
  • Application: Nov 7, 2008
    US US 26666608 A
  • Priority: Nov 7, 2008
    US US 26666608 A
  • Priority: Mar 25, 2005
    US US 8872505 A
  • Priority: Mar 25, 2004
    US US 55606104 P

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