{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_7666608_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"107-118-978-197-675","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8906,"type":"PATENT","title":"Univ Pennsylvania Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":24238,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8221,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search applicants and owners= \"Univ Pennsylvania\", \"Pennsylvania Univ\", \"Univ Penns*\", \"Penn* Univ\", \"University of Pennsylvania\", \"Pennsylvania University\".
Select more for logical variants
Add to collection
Total patent:
Search applicants and owners= \"Univ Pennsylvania\", \"Pennsylvania Univ\", \"Univ Penns*\", \"Penn* Univ\", \"University of Pennsylvania\", \"Pennsylvania University\".
Select more for logical variants
Add to collection
Total patent:
determining the ratio of protein expression levels of serum amyloid A1 (SAA1) gene and serum amyloid A2 (SAA2) gene in a clinical sample from said subject and comparing said ratio to a predetermined control ratio,\n
wherein said SAA1 gene is a steroid responsive gene and said SAA2 gene is a steroid non-responsive gene,\n
wherein said control ratio is the ratio of protein expression levels of said SAA1 gene and said SAA2 gene in either a nonresponsive subject or in a responsive subject, and\n
wherein said subject is steroid responsive if said ratio is higher than the control ratio for a non-responsive subject or is similar to the control ratio for a responsive subject."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein said protein expression levels are determined using at least one of the methods selected from the group consisting of an ELISA assay, Western Blot, Mass Spectrophotometry, and Fluorescence Activated Cell Sorting."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, further comprising monitoring the steroid responsiveness over time to detect a change in responsiveness."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, further comprising administering to the sample at least one of the members selected from the group consisting of at least one pro-inflammatory mediator and at least one anti-inflammatory mediator."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 4, comprising administering tumor necrosis factor (TNF-α)."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 4, comprising administering one or more anti-inflammatory mediators selected from the group consisting of interleukin 1 receptor antagonist (IL-1RA), tumor necrosis factor receptor antagonist (TNF-RA), soluble TNF receptors, anti-TNF antibodies, and anti-TNF-RA antibodies."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, further comprising the step of administering one or more cytokines, chemokines, interferons or hormones to said clinical sample."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, further comprising the step of administering a compound selected from the group consisting of interleukin-8 (IL-8) and vasoactive intestinal peptide (VIP)."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein said sample comprises a fluid selected from the group consisting of blood, serum, plasma, cerebrospinal fluid, ascites fluid, synovial fluid, fluid harvested from a site of inflammation, fluid harvested from a pooled collection site, saliva, semen and bronchial lavage."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein said sample comprises nucleated cells."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 10, wherein said sample comprises cells selected from the group consisting of monocytes, macrophages, neutrophils, T-cells, B-cells, basophils, fibroblasts, endothelial cells and epithelial cells."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein said sample comprises buccal cells."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["A method for determining steroid responsiveness in a subject undergoing steroid treatment, comprising:\n
comparing the protein expression level ratio of SAA1 gene and SAA2 gene in a clinical sample obtained from said subject prior to steroid administration with the expression level ratio of said SAA1 gene and said SAA2 gene in a clinical sample obtained from said subject after administration of a steroid,\n
wherein said SAA1 gene is a steroid responsive gene and said SAA2 gene is a steroid non-responsive gene, and wherein a pre-administration ratio that is less than the post-administration ratio is indicative of steroid responsiveness in said subject, and\n
wherein a pre-administration ratio that is greater than or the same as the post-administration ratio is indicative of steroid non-responsiveness in said subject."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["A method for determining steroid responsiveness in a clinical sample, comprising:\n
comparing the protein expression level ratio of SAA1 gene and SAA2 gene in said clinical sample obtained prior to in vitro exposure of said sample to a steroid with the protein expression level ratio of said SAA1 gene and said SAA2 gene in said clinical sample obtained after in vitro exposure of said sample to a steroid,\n
wherein said SAA1 gene is a steroid responsive gene and said SAA2 gene is a steroid non-responsive gene, and wherein a pre-exposure ratio that is less than the post-exposure ratio is indicative of steroid responsiveness in said sample, and\n
wherein a pre-exposure ratio that is greater than or the same as than the post-exposure ratio is indicative of steroid non-responsiveness in said sample."],"number":14,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}