Increased Production Of Secreted Proteins By Recombinant Eukaryotic Cells

Abstract

Described herein are methods for increasing the amount of protein secreted by a cell. In one case, a cell is provided which contains a heterologous nucleic acid encoding a protein having unfolded protein response modulating activity and a heterologous nucleic acid encoding a protein of interest to be secreted. In one case, the protein having unfolded protein response modulating activity is selected from the proteins selected from the group consisting of HAC1, PTC2 and IRE1. The protein of interest can be any secreted protein such as a therapeutic or an industrial enzyme. For example the protein can be selected from the group consisting of lipase, cellulase, endo-glucosidase H, protease, carbohydrase, reductase, oxidase, isomerase, transferase, kinase, phosphatase, alpha-amylase, glucoamylase, lignocellulose hemicellulase, pectinase and ligninase.


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Document History
  • Publication: Feb 16, 2010
  • Application: Sep 15, 2003
    US US 66345003 A
  • Priority: Sep 15, 2003
    US US 66345003 A
  • Priority: Mar 23, 2001
    US US 81627701 A
  • Priority: Mar 24, 2000
    US US 53469200 A

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