{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_7659119_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"124-720-537-768-91X"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10709,"type":"PATENT","title":"Rockefeller University Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":4213,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8226,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: \"Rockefeller University\"; \"Univ* Rockefeller\"; \"Univ* Rockef*\"
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 4020
Search Applicants and Owners separately: \"Rockefeller University\"; \"Univ* Rockefeller\"; \"Univ* Rockef*\"
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 4020
(a) culturing a population of peripheral blood mononuclear cells comprising pluripotential cells which are CD14 positive and substantially CD83 negative with at least one cytokine to produce a population of immature dendritic cells characterized as substantially CD14 and CD83 negative; and\n
(b) culturing said substantially CD83 negative immature dendritic cells in the presence of a composition selected from the group consisting of fixed Staphylococcus aureus (SACS), peripheral blood mononuclear cell conditioned medium, and monocyte conditioned medium, in an amount and for a time sufficient for said immature dendritic cells to express characteristics of mature dendritic cells, wherein said characteristics of mature dendritic cells include an increase in CD83 and CD11c expression, a decrease in CD68 expression, and persistence of non-adherence for three days following removal of said composition from said culture of dendritic cells."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1, wherein the composition is monocyte conditioned medium."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1, wherein the cytokine is a combination of GM-CSF and either one or both of IL-4 and IL-13."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 3, wherein said pluripotential cells are human."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 4, wherein said pluripotential cells are obtained from human blood."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 5, wherein the cytokine is a combination of GM-CSF and IL-4."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said composition is peripheral blood mononuclear cell conditioned medium."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said composition is macrophage conditioned medium."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said composition is fixed Staphylococcus aureus (SACS)."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said immature dendritic cells are obtained by culturing a population of peripheral blood mononuclear cells with at least one cytokine in medium which does not contain fetal calf serum."],"number":10,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}