Cytotoxin-based Biological Containment


Method of conditionally controlling the survivability of a recombinant cell population and of containing such cells to an environment or containing replicons to a host cell is based on the use of proteic killer systems including E. coli relBE locus and similar systems found in Gram-negative and Gram-positive bacteria and Archae. Such systems are generally based on a cytotoxin polypeptide and an antitoxin or antidote polypeptide that in contrast to the cytotoxin is degradable by proteases. The recombinant cells are useful as vaccines, pollutant degrading organisms or as biological pest control organism e.g. expressing B. thuringiensis crystalline proteins.

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Document History
  • Publication: Sep 29, 2009
  • Application: Aug 10, 2007
    US US 83745607 A
  • Priority: Aug 10, 2007
    US US 83745607 A
  • Priority: Nov 10, 2006
    US US 55885606 A
  • Priority: Dec 18, 2001
    US US 70013001 A
  • Priority: May 7, 1999
    DK DK 9900258 W
  • Priority: May 12, 1998
    US US 8506798 P
  • Priority: May 7, 1998
    DK DK 62798 A

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