{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_7579146_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"010-484-341-690-449","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10801,"type":"PATENT","title":"Boston University - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":4398,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8266,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: \"Boston Univ*\"
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 3218
Search Applicants and Owners separately: \"Boston Univ*\"
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 3218
providing a first precursor nucleic acid molecule including a first double stranded portion flanked by first and second single-stranded overhangs;\n
providing a second precursor nucleic acid molecule including a second double-stranded portion flanked by third and fourth single-stranded overhangs, the third single-stranded overhang being complementary with the second overhang of the first precursor nucleic acid molecule;\n
providing a third precursor nucleic acid molecule including a third double-stranded portion flanked by fifth and sixth single-stranded overhangs, the fifth overhang being complementary with the fourth overhang of the second precursor nucleic acid molecule and the sixth overhang being complementary with the first overhang of the first nucleic acid molecule; and\n
combining the first, second, and third precursor nucleic acid molecules together in a single reaction under conditions that allow for hybridization of the complementary overhangs, so that a new nucleic acid molecule is generated;\n
wherein at least one of the precursor nucleic acid molecules is generated by extending first and second primers in a polymerase-mediated extension reaction, at least one of the first and second primers including at least one base-containing residue, which base-containing residue is not used as a template by the polymerase employed in the polymerase-mediated extension reaction, but which is capable of being used as a template by a different polymerase."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, further comprising a step of:\n
ligating the hybridized precursor nucleic acid molecules together."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2, wherein the step of ligating comprises ligating in vitro."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2, wherein the step of ligating comprises ligating in vivo."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein at least one of the steps of providing a precursor nucleic acid molecule comprises:\n
extending first and second primers in a polymerase-mediated extension reaction, at least one of the primers including at least one residue that renders the primer susceptible to cleavage under conditions in which DNA is not normally susceptible to cleavage."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["A method of generating a protein coding gene, the method comprising steps of:\n
providing a plurality of first precursor nucleic acid molecules, each first precursor nucleic acid molecule including a first double stranded portion flanked by at least a one first single-stranded overhang and having a nucleotide sequence that encodes a first open reading frame;\n
providing a plurality of second precursor nucleic acid molecules, each of which includes a second double-stranded portion flanked by at least one second single-stranded overhang, at least part of which is complementary to at least part of the first single-stranded overhang, wherein each such second precursor nucleic acid molecule has a nucleotide sequence that encodes a second open reading frame;\n
admixing the first and second pluralities of nucleic acid molecules together under conditions that allow for hybridization of the complementary overhangs, so that a plurality of new nucleic acid molecules is generated, wherein each such generated new nucleic acid molecule has a nucleotide sequence that encodes a third open reading frame comprising both of the first and second open reading frames\n
wherein at least one of the precursor nucleic acid molecules in at least one of the provided pluralities is generated by extending first and second primers in a polymerase-mediated extension reaction, at least one of the first and second primers including at least one base-containing residue, which base-containing residue is not used as a template by the polymerase employed in the polymerase-mediated extension reaction, but which is capable of being used as a template by a different polymerase."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6 wherein the second double-stranded portion of each of the second precursor nucleic acid molecules is identical, and wherein at least two second precursor nucleic acid molecules differ from each other at one or more positions in the second single-stranded portion."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, wherein the second single-stranded portion of each of the second precursor nucleic acid molecules is identical, and wherein the second double-stranded portions of each of the second precursor nucleic acid molecules differ from one another."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 8, wherein the open reading frames encoded by the second double-stranded portions each encode a functional domain of a protein."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9, wherein the open reading frames encoded by the second double-stranded portions each encode a different version of the same functional domain."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9, wherein the second double-stranded portions of each of the second precursor nucleic acid molecules are substantially similar, but non-identical to one another."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9 or 11, wherein the second double-stranded portions of each of the second precursor nucleic acid molecules are identical lengths."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, further comprising a step of ligating the hybridized precursor nucleic acid molecules together."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 13, wherein the step of ligating comprises ligating in vitro."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 13, wherein the step of ligating comprises ligating in vivo."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6 wherein the third open reading frame encodes at least part of a protein selected from the group consisting of: animal fatty acid synthases, polyketide synthases, peptide synthetases and terpene synthases."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, further comprising the step of providing a plurality of third precursor nucleic acid molecules, each third precursor nucleic acid molecule including a third double-stranded portion flanked by at least a third single-stranded overhang, wherein each such third precursor nucleic acid molecule has a nucleotide sequence that encodes a third open reading frame, and wherein the third single-stranded overhang is complementary to either the first single-stranded overhang or the second single-stranded overhang."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, wherein the first, second and third pluralities of precursor nucleic acid molecules are admixed simultaneously."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, wherein the first, second and third pluralities of precursor nucleic acid molecules are admixed sequentially."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["A method of generating a protein coding gene, the method comprising steps of:\n
providing at least two collections of precursor nucleic acid molecules, wherein each of said collections comprises at least two nucleic acid molecules that each contain a nucleotide sequence that encodes an open reading frame;\n
selecting an individual nucleic acid molecule, or portion of a nucleic acid molecule, from each of said collections; and\n
admixing the selected nucleic acid molecules or portions together under conditions that allow for hybridization of the complementary overhangs, so that a new nucleic acid molecules is generated that contains a nucleotide sequence that encodes an open reading frame comprising the open reading frames of each of the selected nucleic acid molecules\n
wherein at least one of the selected nucleic acid molecules is generated by extending first and second primers in a polymerase-mediated extension reaction, at least one of the first and second primers including at least one base-containing residue, which base-containing residue is not used as a template by the polymerase employed in the polymerase-mediated extension reaction, but which is capable of being used as a template by a different polymerase."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 20, wherein the open reading frames encoded by the nucleic acid molecules of one of the collections encode a functional domain of a protein."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21, wherein the open reading frames encoded by the nucleic acid molecules of one of the collections encode a different version of the same functional domain."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21, wherein the open reading frames encoded by the nucleic acid molecules of one of the collections are substantially similar, but non-identical to one another."],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21 or 23, wherein the open reading frames encoded by the nucleic acid molecules of one of the collections are identical lengths."],"number":24,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 20, further comprising a step of ligating the hybridized precursor nucleic acid molecules together."],"number":25,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 25, wherein the step of ligating comprises ligating in vitro."],"number":26,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 25, wherein the step of ligating comprises ligating in vivo."],"number":27,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 20 wherein the new nucleic acid molecule generated comprises an open reading frame encoding a protein selected from the group consisting of: animal fatty acid synthases, polyketide synthases, peptide synthetases and terpene synthases."],"number":28,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 20, wherein the selected nucleic acid molecules or portions are admixed simultaneously."],"number":29,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 20, wherein the selected nucleic acid molecules or portions are admixed sequentially."],"number":30,"annotation":false,"claim":true,"title":false},{"lines":["A method of generating a protein coding gene, the method comprising steps of:\n
providing a first plurality of first precursor nucleic acid molecules, each first precursor nucleic acid molecule including a first double stranded portion flanked by at least a first single-stranded overhang and having a nucleotide sequence that encodes a first open reading frame;\n
providing a second precursor nucleic acid molecule that includes a second double stranded portion flanked by at least a second single-stranded overhang, the second single-stranded overhang being complementary to the first single-stranded overhang of the plurality of precursor nucleic acid molecules wherein the second precursor nucleic acid molecule has a nucleotide sequence that encodes a second open reading frame;\n
admixing the plurality of nucleic acid molecules and the second nucleic acid molecule together in a single reaction under conditions that allow for hybridization of the complementary overhangs, so that a plurality of new nucleic acid molecules is generated wherein each such generated new nucleic acid molecule has a nucleotide sequence that encodes a third open reading frame comprising both of the first and second open reading frames\n
wherein at least one of the first precursor nucleic acid molecules in the first plurality or the second precursor nucleic acid molecule is generated by extending first and second primers in a polymerase-mediated extension reaction, at least one of the first and second primers including at least one base-containing residue, which base-containing residue is not used as a template by the polymerase employed in the polymerase-mediated extension reaction, but which is capable of being used as a template by a different polymerase."],"number":31,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 31 wherein the first double-stranded portion of each of the first precursor nucleic acid molecules is identical, and wherein at least two second precursor nucleic acid molecules differ from each other at one or more positions in the second single-stranded portion."],"number":32,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 31, wherein the first single-stranded portion of each of the second precursor nucleic acid molecules is identical, and wherein the first double-stranded portions of each of the second precursor nucleic acid molecules differ from one another."],"number":33,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 33, wherein the open reading frames encoded by the first double-stranded portions each encode a functional domain of a protein."],"number":34,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 34, wherein the open reading frames encoded by the first double-stranded portions each encode a different version of the same functional domain."],"number":35,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 34, wherein the first double-stranded portions of each of the first precursor nucleic acid molecules are substantially similar, but non-identical to one another."],"number":36,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 34 or 36, wherein the first double-stranded portions of each of the first precursor nucleic acid molecules are identical lengths."],"number":37,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 31, further comprising a step of ligating the hybridized precursor nucleic acid molecules together."],"number":38,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 38, wherein the step of ligating comprises ligating in vitro."],"number":39,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 38, wherein the step of ligating comprises ligating in vivo."],"number":40,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 31 wherein the new nucleic acid molecules generated comprise open reading frames encoding proteins selected from the group consisting of: animal fatty acid synthases, polyketide synthases, peptide synthetases and terpene synthases."],"number":41,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 31, further comprising the step of providing a plurality of third precursor nucleic acid molecules, each third precursor nucleic acid molecule including a third double-stranded portion flanked by at least a third single-stranded overhang, wherein each such third precursor nucleic acid molecule has a nucleotide sequence that encodes a third open reading frame, and wherein the third single-stranded overhang is complementary to either the first single-stranded overhang or the second single-stranded overhang."],"number":42,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 42, wherein the first and third pluralities of precursor nucleic acid molecules and the second precursor nucleic acid molecules are admixed simultaneously."],"number":43,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 42, wherein the first and third pluralities of precursor nucleic acid molecules and the second precursor nucleic acid molecules are admixed sequentially."],"number":44,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 31, further comprising the step of providing a third precursor nucleic acid molecule that includes a third double-stranded portion flanked by at least a third single-stranded overhang, wherein the third precursor nucleic acid molecule has a nucleotide sequence that encodes a third open reading frame, and wherein the third single-stranded overhang is complementary to either the first single-stranded overhang or the second single-stranded overhang."],"number":45,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 45, wherein the first plurality of precursor nucleic acid molecules and the second and third precursor nucleic acid molecules are admixed simultaneously."],"number":46,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 45, wherein the first plurality of precursor nucleic acid molecules and the second and third precursor nucleic acid molecules are admixed sequentially."],"number":47,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}