{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_7521177_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"198-707-162-146-302","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11757,"type":"PATENT","title":"Colorado State University Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":7894,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8396,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: colorado state uni*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1775
Search Applicants and Owners separately: colorado state uni*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1775
(a) contacting the sample with a flavivirus antigen,\n
which flavivirus antigen is an immunogenic flavivirus antigen produced by expressing a transcriptional unit encoding a Japanese encephalitis virus (JEV) signal sequence and an immunogenic flavivirus antigen of a flavivirus other than JEV, wherein the transcriptional unit directs the synthesis of the immunogenic flavivirus antigen,\n
under conditions whereby an antigen/antibody complex can form; and\n
(b) detecting an increase in antigen/antibody complex formation in the sample relative to a negative control, thereby detecting a flavivirus antibody in the sample."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the sample is obtained from a subject."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the transcriptional unit encodes an engineered JEV signal sequence."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 3, wherein the engineered JEV signal sequence comprises SEQ ID NO:14."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the immunogenic flavivirus antigen of a flavivirus other than JEV is of a flavivirus selected from the group consisting of yellow fever virus, dengue serotype 1 virus, dengue serotype 2 virus, dengue serotype 3 virus, dengue serotype 4 virus, St. Louis encephalitis virus, Powassan virus and West Nile virus."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the immunogenic flavivirus antigen of a flavivirus other than JEV is selected from the group consisting of an M protein of a flavivirus, an E protein of a flavivirus, both an M protein and an E protein of a flavivirus, a portion of an M protein of flavivirus, a portion of an E protein of a flavivirus and both a portion of an M protein of a flavivirus and a portion of an E protein of a flavivirus or any combination thereof."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6, wherein the immunogenic flavivirus antigen of a flavivirus other than JEV is both the M protein and the E protein of a flavivirus."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the transcriptional unit comprises a control sequence disposed appropriately such that it operably controls the synthesis of the immunogeic flavivirus antigen."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 8, wherein the control sequence is the cytomegalovirus immediate early promoter."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, comprising a Kozak consensus sequence located at a translational start site for a polypeptide comprising the immunogenic flavivirus antigen encoded by the transcriptional unit."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the transcriptional unit comprises a poly-A terminator."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the sample is a fluid sample or a tissue sample."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12, wherein the fluid sample comprises at least one of cerebrospinal fluid, blood, bile, plasma, serum, saliva, urine, sputum and mucus."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the sample is contacted with the flavivirus antigen in an immunofluorescence assay (IFA), an enzyme linked immunosorbent assay (ELISA), an immunoblotting assay or a microagglutination assay."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, further comprising contacting the antigen/antibody complex with a monoclonal antibody specific for at least one flavivirus."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the flavivirus antigen is bound to a substrate."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the sample is at least partially purified prior to contacting with the flavivirus antigen."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 3, wherein the engineered JEV signal sequence comprises SEQ ID NO:27."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["A method of detecting a flavivirus antibody in a sample, comprising:\n
(a) contacting the sample with a flavivirus antigen,\n
which flavivirus antigen is an immunogenic flavivirus antigen produced by expressing a transcriptional unit encoding an engineered Japanese encephalitis virus (JEV) signal sequence comprising SEQ ID NO: 14, and an immunogenic flavivirus antigen comprising an M protein and an E protein of a flavivirus selected from the group consisting of yellow fever virus, dengue serotype 1 virus, dengue serotype 2 virus, dengue serotype 3 virus, dengue serotype 4 virus, St. Louis encephalitis virus, Powassan virus and West Nile virus, wherein the transcriptional unit directs the synthesis of the immunogenic flavivirus antigen,\n
under conditions whereby an antigen/antibody complex can form; and\n
(b) detecting an increase in antigen/antibody complex formation in the sample relative to a negative control, thereby detecting a flavivirus antibody in the sample."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["A method of detecting a flavivirus antibody in a sample, comprising:\n
(a) contacting the sample with a flavivirus antigen,\n
which flavivirus antigen is an immunogenic flavivirus antigen produced by expressing a transcriptional unit encoding an engineered Japanese encephalitis virus (JEV) signal sequence comprising SEQ ID NO: 27, and an immunogenic flavivirus antigen comprising an M protein and an E protein of a flavivirus selected from the group consisting of yellow fever virus, dengue serotype 1 virus, dengue serotype 2 virus, dengue serotype 3 virus, dengue serotype 4 virus, St. Louis encephalitis virus, Powassan virus and West Nile virus, wherein the transcriptional unit directs the synthesis of the immunogenic flavivirus antigen,\n
under conditions whereby an antigen/antibody complex can form; and\n
(b) detecting an increase in antigen/antibody complex formation in the sample relative to a negative control, thereby detecting a flavivirus antibody in the sample."],"number":20,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}