{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_7510831_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"030-991-782-396-220"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11844,"type":"PATENT","title":"University of Kansas Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":8584,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8456,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: univ* AND Kansas. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1781
Search Applicants and Owners separately: univ* AND Kansas. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1781
(a) providing a host cell transformed with an expression vector comprising a polynucleotide which codes for a enzyme having phytase activity having the amino acid sequence with at least 90% sequence identity over the entire length of SEQ ID NO:5, wherein the phosphate binding region has a sequence (V/L)L(A/S)RHGAR;\n
(b) culturing said transformed host cell under culture conditions for production of said enzyme; and\n
(c) recovering said enzyme wherein said phytase hydrolyzes phytate to inositol in a phytase assay."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said host cell is an Aspergillus species."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said host cell is a Trichoderma species."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["A purified enzyme having phytase activity, produced by the method of claim 1, said enzyme having an amino acid sequence with at least 90% sequence identity to SEQ ID NO:5, wherein the phosphate binding region has a sequence (V/L)L(A/S)RHGAR."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1, wherein the polynucleotide codes for an enzyme having phytase activity having at least 95% sequence identity to SEQ ID NO:5."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1, wherein the polynucleotide has at least 85% sequence identity to the nucleotide sequence disclosed in SEQ ID NOS: 1, 2 or 3."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["An enzyme composition comprising the purified enzyme of claim 4."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2, wherein the Aspergillus species is A. niger. "],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, wherein the Trichoderma species is T. reesei. "],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 5, wherein the polynucleotide codes for an enzyme comprising SEQ ID NO:5."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["An enzyme composition comprising the recovered enzyme of claim 1."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein at least one of the domains selected from the group consisting of: Box 2, Box 4, box 5, and Box 6 in SEQ ID NO:5 is conserved."],"number":12,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}