Cultured Xenopus Laevis Cell Lines Expressing Mutant Adenomatous Polyposis Coli Gene


Using protein visualization techniques, cell lines stably expressing GFP-fused full-length and mutant APCs were obtained from cultured Xenopus laevis renal epithelial A6 cells. The use of these cells showed that mutant APCs, whose C-terminal region is absent, induced piling up of cells. Furthermore, piled up cells from mutant APC-expressing cell lines were proven to maintain the intercellular adhesive structure, representing a phenomenon similar to polyp formation in individual organisms (mice).

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Document History
  • Publication: May 13, 2008
  • Application: Sep 7, 2005
    US US 52562105 A
  • Priority: Aug 19, 2003
    JP 0310434 W
  • Priority: Aug 22, 2002
    JP 2002241487 A

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