Method For Generating Diversity


The invention relates to a method for preparing an antibody-producing cell line capable of directed constitutive hypermutation of a specific nucleic acid region, comprising the steps of: a) screening a clonal cell population for V gene diversity; b) isolating one or more cells which display V gene diversity and comparing the rate of accumulation of mutations in the V genes and other genes of the selected cells; and c) selecting a cell in which the rate of V gene mutation exceeds that of other gene mutation.

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Document History
  • Publication: May 6, 2008
  • Application: Dec 11, 2003
    US US 73353203 A
  • Priority: Dec 11, 2003
    US US 73353203 A
  • Priority: Jun 11, 2002
    GB GB 0202688 W
  • Priority: May 15, 2002
    US US 14650502 A
  • Priority: Jun 11, 2001
    US US 87981301 A

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