{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_7364750_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"127-543-062-897-607","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10737,"type":"PATENT","title":"The University of British Columbia - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":7923,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8233,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:\"univ* briti* colum*\".
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 7363
Search Applicants and Owners separately:\"univ* briti* colum*\".
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 7363
(a) a eukaryotic promoter and a first RNA polymerase promoter operably linked to a nucleic acid encoding a secretable RNA polymerase (sRNAP) comprising a RNA polymerase and a secretion domain; and\n
(b) a second RNA polymerase promoter operably linked to a nucleic acid encoding a product of interest, wherein the sRNAP enters the cytoplasm of a cell and carries out template dependent synthesis of RNA."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 1, wherein said RNA polymerase is a non-host RNA polymerase."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 1, wherein said RNA polymerase is a member selected from the group consisting of: a phagemid RNA polymerase, a prokaryotic RNA polymerase, an archaebacterial RNA polymerase, a plant RNA polymerase, a fungal RNA polymerase, a eukaryotic RNA polymerase, a viral RNA polymerase, mitochondrial RNA polymerase, and a chloroplast RNA polymerase."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 3, wherein said phagemid RNA polymerase is a member selected from the group consisting of: a SP6 RNA Polymerase, a T7 RNA Polymerase, a K11 RNA Polymerase, and a T3 RNA Polymerase."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 1, wherein said product of interest is a member selected from the group consisting of: a protein, a nucleic acid, an antisense nucleic acid, ribozymes, tRNA, snRNA, and an antigen."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 1, wherein said product of interest encodes a protein selected from the group consisting of:\n
a herpes simplex virus thymidine kinase (HSV-TK), a cytosine deaminase, a xanthine-guaninephosphoribosyl transferase, a purine nucleoside phosphorylase, a carboxylesterase, a deoxycytidine kinase, a nitroreductase, a thyrnidine phosphorylase, and a cytochrome P450 2B1."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 1, wherein said product of interest encodes a protein selected from the group consisting of: p53, a retinoblastoma susceptibility gene product (RB1), a calmodulin-dependent serine threonine (DAP kinase), p16, a protein from an alternative reading frame of p16 (ARF), an adenomatous polyposis coli gene product (APC), neurofibromin, a phosphatase and tensin homologue deleted from chromosome 10 (PTEN), a Wilms= tumor gene product (WT1), a neurofibromatosis type 1 gene product (NF1), and a von Hippel-Lindau tumor suppressor gene product (VHL)."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 1, wherein said product of interest encodes a protein selected from the group consisting of: angiostatin, endostatin, and vascular endothelial growth factor (VEGF)-R2."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 1, wherein said product of interest encodes apoptin."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 1, wherein said product of interest encodes a protein selected from the group consisting of: IL-2, IL-3, IL-4, IL-6, IL-7, IL-10, IL-12, IL-15, IFN-α, IFN-β, IFN-γ, TNF-α, GM-CSF, G-CSF, and Fms-like tyrosine kinase 3 (Flt3)-Ligand."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 1, wherein said product of interest is a protein selected from the group consisting of: a restriction endonuclease, a single chain antibody, a peptide hormone, erythropoietin (EPO), and single-chain insulin."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 1, wherein said eukaryotic promoter is a member selected from a group consisting of: a cytomegalovirus promoter, a von Willebrand factor (vWf) promoter, a Clara cell secretory protein (CCSP/UG) promoter, an osteoblast-specific osteocalcin promoter, an albumin promoter, a muscle creatine kinase (MCK) promoter, a mucin-1 (Muc-1) promoter, a carcinoembryonic antigen (CEA) promoter, a prostate specific antigen (PSA) promoter, an epidermal growth factor receptor (HER-2) promoter, a Myc promoter, a L-plastin promoter, an alpha-fetoprotein (AFP) promoter, a hypoxia-responsive element (HIRE) promoter, an early growth response (egr-1) promoter, a multidrug resistance (mdr-1) promoter, a heat shock protein 70 (hsp70) promoter, a tetracycline induced promoter, a simian virus 40 (SV40) promoter, an alcohol dehydrogenase (ADH1) promoter, a GAL4 promoter, a LexA promoter, a rous sarcoma virus (RSV) promoter, a human imniunodeficiency virus (HIV) promoter, and a simian foamy virus (SFV) promoter."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["A lipid-nucleic acid composition comprising:\n
a nucleic acid-lipid particle comprising a lipid portion and a nucleic acid portion,\n
wherein said nucleic acid portion comprises a secretable RNA polymerase expression cassette comprising:\n
(a) a eukaryotic promoter and a first RNA polymerase promoter operably linked to a nucleic acid encoding a secretable RNA polymerase comprising a RNA polymerase and a secretion domain; and\n
(b) a second RNA polymerase promoter operably linked to a nucleic acid encoding a product of interest."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The nucleic acid of claim 13, wherein a RNA polymerase promoter is operably linked to said nucleic acid encoding a secretable RNA polymerase."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The lipid-nucleic acid composition of claim 13, wherein said nucleic acid-lipid particle is a serum-stable nucleic acid-lipid particle comprising a nucleic acid fuily encapsulated within said lipid portion."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The lipid-nucleic acid composition of claim 13, wherein said lipid portion comprises a protonatable lipid having a pKa in a range of about 4 to about 11."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The lipid-nucleic acid composition of claim 13, wherein said lipid portion comprises a cationic lipid."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The lipid-nucleic acid composition of claim 17, wherein said cationic lipid is a member selected from the group consisting of DODAC, DODAP, DODMA, DOTAP, DOTMA, DC-Chol, DMRIE, and DSDAC."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 1, wherein said secretion domain is an importation competent signal peptide."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 1, wherein said secretion domain is a protein transduction domain."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 20, wherein said protein transduction domain is a TAT polypeptide."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 21, wherein said TAT polypeptide comprises the amino acid sequence X1-X2-X3-X4-X5-X6-X7-X8-X9-Arg-X10, wherein\n
X1 is Tyr or Ala;\n
X2 is Gly or Ala;\n
X3, X6, X7, X9 and X10 are Arg or Ala; and\n
X8 is Gln, Arg or Ala."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 22, wherein said TAT polypeptide comprises SEQ ID NO:1."],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["The lipid-nucleic acid composition of claim 13, wherein said secretion domain is an importation competent signal peptide."],"number":24,"annotation":false,"claim":true,"title":false},{"lines":["The lipid-nucleic acid composition of claim 13, wherein said secretion domain is a protein transduction domain."],"number":25,"annotation":false,"claim":true,"title":false},{"lines":["The lipid-nucleic acid composition of claim 25, wherein said protein transduction domain is a TAT polypeptide."],"number":26,"annotation":false,"claim":true,"title":false},{"lines":["The lipid-nucleic acid composition of claim 26, wherein said TAT polypeptide comprises the amino acid sequence X1-X2-X3-X4-X5-X6-X7-X8-X9-Arg-X10, wherein\n
X1 is Tyr or Ala;\n
X2 is Gly or Ala;\n
X3, X6, X7, X9 and X10 are Arg or Ala; and\n
X8 is Gln, Arg or Ala."],"number":27,"annotation":false,"claim":true,"title":false},{"lines":["The lipid-nucleic acid composition of claim 27, wherein said TAT polypeptide comprises SEQ ID NO:1."],"number":28,"annotation":false,"claim":true,"title":false},{"lines":["The expression cassette of claim 1, wherein the RNA polymerase is a T7 RNA polymerase."],"number":29,"annotation":false,"claim":true,"title":false},{"lines":["The lipid-nucleic acid composition of claim 13, wherein the RNA polymerase is a T7 RNA polymerase."],"number":30,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}