{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_7326770_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"005-734-306-087-466"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11650,"type":"PATENT","title":"University of Alberta - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":3172,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8316,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
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Add to collection. Total patents: 3178
contacting the isolated fucosyltransferase protein of claim 1 with a mixture comprising a donor substrate comprising a fucose residue, and an acceptor substrate on a glycolipid, under conditions where the fucosyltransferase catalyzes the transfer of the fucose residue from a donor substrate to the acceptor substrate on the glycolipid, thereby producing a fucosylated glycolipid."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["A method of making a fucosylated oligosaccharide, the method comprising:\n
contacting the isolated fucosyltransferase of claim 1 with a mixture comprising a donor substrate comprising a fucose residue, and an acceptor substrate comprising a sugar or oligosaccharide, under conditions where the fusion protein catalyzes the transfer of a fucose residue from the donor substrate to the acceptor substrate, thereby producing a fucosylated oligosaccharide."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 7, wherein the method further comprises a step of purifying the fucosylated oligosaccharide."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 7, wherein a donor substrate is GDP-fucose."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 7, wherein the fucosyltransferase comprises an amino acid tag."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 7, wherein an acceptor substrate comprises a member selected from N-acetylglucosamine and glucose."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 7, wherein the acceptor substrate is Lacto-N-neo-Tetraose (LNnT)."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 12, wherein the fucosylated oligosaccharide is Lacto-N-Fucopentaose III (LNFP III)."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 7, wherein the mixture further comprises lactose, a β-1,3-N-acetylglucosaminyltransferase, and a β-1,4-galactosyltransferase."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 14, wherein the fucosylated oligosaccharide is Lacto-N-Fucopentaose III (LNFP III)."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 14, wherein the β-1,3-N-acetylglucosaminyltransferase is a bacterial enzyme."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 16, wherein the β-1,3-N-acetylglucosaminyltransferase is from Neisseria gonococcus."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 14, wherein the β-1,4-galactosyltransferase is a bacterial enzyme."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 18, wherein the β-1,4-galactosyltransferase is from Neisseria gonococcus."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["A method for producing a fucosylated glycoprotein, the method comprising:\n
contacting a host cell extract comprising a fucosyltransferase protein having greater than 90% identity to the full-length of SEQ ID NO:16, with a mixture comprising a donor substrate comprising a fucose residue, and an acceptor substrate on a glycoprotein, under conditions where the fucosyltransferase catalyzes the transfer of the fucose residue from a donor substrate to the acceptor substrate on the glycoprotein, thereby producing a fucosylated glycoprotein, wherein the host cell extract is heterologous to the fucosyltransferase protein."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the polypeptide comprises an amino acid sequence having greater than 95% identity to the full-length of SEQ ID NO:16."],"number":21,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the polypeptide comprises SEQ ID NO:16."],"number":22,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the polypeptide further comprises an amino acid tag."],"number":23,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the method further comprises a step of purifying the fucosylated glycoprotein."],"number":24,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the acceptor substrate is selected from a glucose residue and an N-acetylglucosamine residue."],"number":25,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein an acceptor substrate on the glycoprotein comprises Galb1-OR, Galb,3/4GlcNAc-OR, NeuAca2,3Galb1,3/4GlcNAc-Or, wherein R is an amino acid, a saccharide, an oligosaccharide, or an aglycon group having at least one carbon atom."],"number":26,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}