{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_7314737_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"120-452-298-009-532","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10809,"type":"PATENT","title":"Leiden University - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":2497,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8272,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: \"Leiden Univ*\"
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1568
Search Applicants and Owners separately: \"Leiden Univ*\"
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1568
introducing the fusion protein into the cell to be changed, which fusion protein comprises a first part A and a second part B, the first part A and second part B derived from different proteins, wherein;\n
the first part A comprises an oligopeptide means for transporting part B through said pore, said means having the 20 most C-terminal amino acids of a bacterial protein transported through said pore, and at least two arginine or lysine amino acids and at least 50% hydrophilic or neutral amino acids; and\n
the second part B comprises a polypeptide capable of exerting a cell-changing activity in the cell to be changed,\n
wherein a C-terminal end of the polypeptide of the second part B is linked to an N-terminal end of the first part A, with the proviso that if the fusion protein comprises a first part A derived from VirE2, the fusion protein does not comprise the 84 N-terminal amino acids of VirE2; and\n
effecting a change in the cell."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the fusion protein is formed by expression in the bacterial transfer system."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the fusion protein is introduced into the cell to be changed without introducing a DNA- or RNA-sequence."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 2, wherein the fusion protein is introduced into the cell to be changed without introducing a DNA- or RNA-sequence."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the introduced fusion protein possesses a recombinase activity."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 2, wherein the introduced fusion protein possesses a recombinase activity."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 3, wherein the introduced fusion protein possesses a recombinase activity."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 4, wherein the introduced fusion protein possesses a recombinase activity."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein a bacterium from the class of Rhizobiaceae is used as the transfer system."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the cell to be modified is selected from the group consisting of i) a plant cell; ii) a yeast cell; and iii) a fungal cell."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["A vector that codes for a fusion protein BA, for use in a bacterial protein transport system comprising a pore and the fusion protein BA, wherein the pore comprises a VirB protein complex and a VirD4 coupling factor, the vector comprising:\n
a nucleic acid sequence coding for the fusion protein BA, wherein the nucleic acid sequence comprises a 5′ first part A linked to a 3′ second part B, wherein the first part A and the second part B encodes an amino acid sequence and the amino acid sequence of part A and part B are derived from different proteins, wherein:\n
the first part A encodes an oligopeptide means for transporting the 3′ second part B through the pore, said means comprising at least a 20 most C-terminal amino acid portion of a bacterial protein transported through said pore, and comprises at least two arginine or lysine amino acids and having at least 50% hydrophilic or neutral amino acids; and\n
the second part B encodes a polypeptide capable of effecting a cell-modifying activity in a cell to be modified, with the proviso that if the nucleic acid sequence comprises a first part A encoding at least the 20 most C-terminal amino acid portion of VirE2, the nucleic acid sequence does not encode a 84 most N-terminal amino acid portion of VirE2."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["A vector set, comprising one or more vectors coding for a bacterial protein transport system, wherein the bacterial protein transport system comprises a fusion protein and a pore comprising a VirB protein complex and a VirD4 coupling factor, wherein the fusion protein comprises:\n
a first amino acid sequence at an N-terminal end of the fusion protein and a second amino acid sequence at a C-terminal end of the fusion protein, wherein the first amino acid sequence and the second amino acid sequence of the fusion protein are derived from different proteins, the first amino acid sequence being capable of exercising a cell-modifying activity in a cell to be modified; and\n
the second amino acid sequence comprising at least the 20 C-terminal amino acids of a bacterial protein, wherein the at least 20 C-terminal amino acids are a means for transporting the first amino acid sequence through the pore and comprises at least two arginine or lysine amino acids and at least 50% hydrophilic or neutral amino acids; with the proviso that if the at least 20 C-terminal amino acids are derived from VirE2, the fusion protein does not comprise a 84 most N-terminal amino acid region of VirE2."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The vector set of claim 12, wherein the second amino acid sequence comprises at least 20 amino acids selected from the group consisting of at least the 20 most C-terminal amino acids of VirF, VirD2, VirE2, VirE3, VirD5 and MobA."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the bacterial protein transported through said pore is selected from the group consisting of VirF, VirD2, VirE2, VirE3, VirD5 and MobA."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The vector of claim 11, wherein the bacterial protein transported through said pore is selected from the group consisting of VirF, VirD2, VirE2, VirE3, VirD5 and MobA."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The vector set of claim 12, wherein the bacterial protein transported through said pore is selected from the group consisting of VirF, VirD2, VirE2, VirE3, VirD5 and MobA."],"number":16,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}