Method Of Effecting A Change In A Cell, And A Vector


A method of effecting a change in a cell using a transfer system which is contacted with the cell to be changed. The transfer system comprises a fusion protein which is transferred into the cell using the VirB/VirD4 transfer system. According to the invention, a fusion protein BA is introduced into the cell to be changed comprising i) as a first part A, an oligopeptide comprising the C-terminal amino acids 1-20 of VirF, VirD2, VirE2, VirE3, VirD5 or MobA, or an analogue thereof and ii) as a second part B, a polypeptide capable of effecting a cell-changing activity in the cell to be changed, wherein the polypeptide is attached with the C-terminal end thereof linked with the N-terminal end of the first part.

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Document History
  • Publication: Jan 1, 2008
  • Application: Nov 19, 2002
    US US 30066602 A
  • Priority: May 21, 2001
    NL NL 0100388 W
  • Priority: May 19, 2000
    NL NL 1015252 A

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