{"search_session":{},"preferences":{"l":"zh","queryLanguage":"zh"},"patentId":"US_7312047_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"064-562-935-107-126","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11669,"type":"PATENT","title":"University of Southampton - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":3699,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8332,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:uni* southamp*
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Add to collection. Total patents: 3701
Search Applicants and Owners separately:uni* southamp*
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Select all patents in the collection and expand by simple families.
Add to collection. Total patents: 3701
(i) providing cultured asthmatic bronchial epithelial cells;\n
(ii) further culturing said cells on a porous support at an air-liquid interface whereby they differentiate in culture and will form an in vitro epithelium, said culturing being in the absence of any added Th2 or proinflammatory cytokine and for a period such that an epithelial barrier is detectable;\n
(iii) adding to the culture, either at the start of culturing or during culturing said test agent whereby the test agent is contacted with the epithelial cells;\n
(iv) and determining at one or more time points after formation of an epithelial barrier is detectable whether said test agent improves epithelial barrier function compared with epithelial barrier function detectable in an identical culture without the test agent."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["A method as claimed in claim 1 wherein said determining is performed by transepithelial electrical resistance measurement."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["A method as claimed in claim 1 wherein said determining is performed after visually observing a continuous monolayer of cells."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["A method as claimed in claim 1 wherein said determining is, performed after attaining full differentiation and maximum epithelial barrier function."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["A method as claimed in claim 1 wherein said determining includes determining epithelial barrier function at one or more time points at 7 days or longer from the start of air-liquid interface culturing."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["A method as claimed in claim 1 wherein the providing of cultured asthmatic bronchial epithelial cells for air-liquid interface culturing in step (i) comprises (a) growing isolated asthmatic bronchial epithelial cells in a primary culturing step, (b) transferring cells to said porous support and (c) performing submerged culturing of the cells on said support to reach 90 to 100% confluence prior to step (ii)."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["A method as claimed in claim 1 wherein said test agent is a coating agent."],"number":7,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}