High Throughput Method For Discovery Of Gene Clusters

Abstract

A method for identifying gene cluster is disclosed. The method may be used for identifying gene clusters involved in the biosynthesis of natural products. A small insert library of DNA fragments of genomic DNA and a large insert library of DNA fragments of genomic DNA are prepared. Fragments in the small insert library are sequenced and compared by homology comparison under computer control to a database containing genes, gene fragments or proteins known to be involved in the biosynthesis of microbial natural products. Fragments having similar structure to genes, gene fragments or proteins known to be involved in the biosynthesis of naturally occurring metabolites are used as probes to screen the large insert library of genomic DNA to detect gene clusters involved in the biosynthesis of microbial natural products.


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Document History
  • Publication: Aug 14, 2007
  • Application: Sep 3, 2002
    US US 23237002 A
  • Priority: Sep 3, 2002
    US US 23237002 A
  • Priority: Jul 26, 2002
    US US 20503202 A
  • Priority: Jun 11, 2002
    US US 16608702 A
  • Priority: May 21, 2002
    US US 15288602 A
  • Priority: Apr 26, 2002
    US US 13213402 A
  • Priority: Apr 17, 2002
    US US 37278902 P
  • Priority: Dec 26, 2001
    US US 34213301 P
  • Priority: Dec 3, 2001
    US US 33460401 P
  • Priority: Oct 15, 2001
    US US 97605901 A
  • Priority: Jul 26, 2001
    US US 30762901 P
  • Priority: Jul 24, 2001
    US US 91081301 A
  • Priority: Jun 11, 2001
    US US 29674401 P
  • Priority: May 21, 2001
    US US 29195901 P
  • Priority: Apr 26, 2001
    US US 28634601 P
  • Priority: Oct 13, 2000
    US US 23992400 P

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