Methods And Compositions Of Enzymatic Cycling Based Assays For Myeloperoxidase

Abstract

The present invention provides a method for assaying myeloperoxidase activity. In the assay, a sample containing myeloperoxidase or suspected of containing myeloperoxidase is contacted with substrate including serine, hydrogen peroxide, and a halide. If myeloperoxidase is present in the sample, serine is converted into glycolaldehyde, which is further converted into glycolate by a glycoaldehyde converting enzyme. The method then utilizes a cycling reaction system between glycolate and glyoxylate to generate a detectable signal that corresponds to the myeloperoxidase activity. Kits for assaying myeloperoxidases based on the same principle are also provided.


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Document History
  • Publication: Mar 27, 2007
  • Application: Aug 11, 2004
    US US 91656304 A
  • Priority: Aug 11, 2004
    US US 91656304 A

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