Bacterial Inosine 5'-monophosphate Dehydrogenase ("impdh") Dna As A Dominant Selectable Marker In Mammals And Other Eukaryotes


The present invention relates to a nucleic acid sequence and its corresponding protein sequence useful as a dominant selectable marker in eukaryotes. More specifically the invention relates to a nucleic acid encoding a bacterial IMPDH gene that has been engineered into a eukaryotic expression vectors, thereby permitting bacterial IMPDH expression in mammalian cells. Bacterial IMPDH expression confers resistance to MPA which can be used as dominant selectable marker in eukaryotes including mammals. The invention also relates to expression vectors and cells that express the bacterial IMPDH gene as well as gene therapies and protein synthesis.

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Document History
  • Publication: Feb 27, 2007
  • Application: May 28, 2004
    US US 85640904 A
  • Priority: May 28, 2004
    US US 85640904 A

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