{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_7148022_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"044-366-941-749-068"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":12009,"type":"PATENT","title":"University of St Andrews Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":1433,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8573,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:Univ* Andrews. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1345
Search Applicants and Owners separately:Univ* Andrews. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1345
i) providing a first component consisting of protein 2C of a picornavirus or a protein 2C homolog from a calicivirus;\n
ii) providing a second component consisting of DAKAP2;\n
iii) exposing said first component to a test substance followed by addition of said second component, or exposing said second component to said test substance followed by addition of said first component, or exposing said first component to said second component followed by said test substance;\n
iv) incubating the mixture obtained in step (iii) for a time period and under conditions suitable to allow interaction between the components and/or the test substance;\n
v) removal of any unassociated first or second component and/or test substance; and\n
vi) detecting the presence, and optionally determining the amount, of associated first and second components."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The assay as claimed in claim 1 which is conducted in vitro."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The assay as claimed in claim 1 wherein either the first or second components is localised on a surface."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The assay as claimed in claim 1 wherein association of said first and second components is detected by using an antibody, a radio-label, fluorescence, or an enzymic-label."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The assay as claimed in claim 1 wherein picornavirus protein 2C and D-AKAP are expressed from a co-expression construct."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The assay as claimed in claim 5 wherein said co-expression construct expresses D-AKAP2 and also expresses picornavirus protein 2C, wherein said protein 2C is present as part of a P2/ P3 polyprotein having a 2A proteinase cleavage site, and wherein said DAKAP2 is linked to the P2/ P3 polyprotein via a 2A proteinase cleavage site."],"number":6,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}