{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_7011975_B1","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"164-607-359-470-26X"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11706,"type":"PATENT","title":"The University of Georgia - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":3798,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8360,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: uni* georgia
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Add to collection. Total patents: 3820
Search Applicants and Owners separately: uni* georgia
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Select all patents in the collection and expand by simple families.
Add to collection. Total patents: 3820
a) obtaining a transgenic cell comprising a heterologous polynucleotide encoding an aminopeptidase N (APN) wherein a functional APN is produced by said cell and localized on the extracellular membrane of said cell, and said APN is able to bind a Bacillus insect toxin protein;\n
b) exposing said cell to a composition suspected of comprising a Bacillus insect toxin; and\n
c) determining if a Bacillus insect toxin is bound to said APN,\n
wherein said polynucleotide is capable of hybridizing with a probe corresponding to the complement of the nucleic acid sequence of SEQ ID NO:2, wherein hybridization is maintained under conditions of 0.1× standard saline sodium phosphate EDTA buffer (SSPE) and 0.1% sodium dodecyl sulfate (SDS) at 65° C. for 15 minutes."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 6 wherein said APN has at least 95% identity with the amino acid sequence of SEQ ID NO:1."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["A method of screening for an aminopeptidase inhibitor wherein said method comprises the steps of:\n
a) obtaining a plurality of transgenic cells wherein each said cell comprises an isolated polynucleotide encoding an aminopeptidase N (APN), wherein a functional APN is produced by said cell and localized on the extracellular membrane of said cell, and said APN is able to bind an aminopeptidase inhibitor;\n
b) exposing said cells to at least one potentially APN-inhibiting compound;\n
c) identifying a compound that binds to said APN; and\n
d) assaying said compound for its ability to inhibit an aminopeptidase,\n
wherein said polynucleotide is capable of hybridizing with a probe corresponding to the complement of the nucleic acid sequence of SEQ ID NO:2, wherein hybridization is maintained under conditions of 0.1× standard saline sodium phosphate EDTA buffer (SSPE) and 0.1% sodium dodecyl sulfate (SDS) at 65° C. for 15 minutes."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 8 wherein said compound binds to an aminopeptidase of an insect."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 8 wherein said compound inhibits an aminopeptidase of an insect."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 8 wherein said APN has at least 95% identity with the amino acid sequence of SEQ ID NO:1."],"number":11,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}