Methods And Compositions For The Differentiation Of Human Preadipocytes Into Adipocytes


The present invention provides methods and compositions for the consistent and quantitative differentiation of human preadipocytes isolated from adipose tissue into adipocytes bearing biochemical, genetic, and physiological characteristics similar to that observed in isolated primary adipocytes. The methods of the invention comprise incubating isolated human preadipocytes, plated at least about 25,000 cells/cm<SUP>2</SUP>, in a medium containing, glucose, a cyclic AMP inducer such as isobutylmethylxanthine or forskolin, a glucocorticoid or glucocorticoid analogue, insulin or an insulin analogue and a PPARgamma agonist or a RXR agonist. The compositions of the invention include media for the differentiation of human preadipocytes, human adipocytes differentiated by the methods of the invention and transfected adipocytes.

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Document History
  • Publication: Feb 21, 2006
  • Application: Jun 1, 2000
    US US 58582100 A
  • Priority: Jun 1, 2000
    US US 58582100 A
  • Priority: Jan 29, 1999
    US US 24002999 A

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