Abstract
An oncogene designated PAX8-PPARgamma1 contains a PAX8 coding region fused to PPARgamma coding region. Molecular characterization of PAX8-PPARgamma1 molecules provides nucleotide and amino acid sequences useful for detection and treatment of certain tumors, particularly thyroid follicular carcinomas.
Claims
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A method of identifying the presence of a PAX8-PPARγ1 or a PPARγ1-PAX8 molecule in a sample, the method comprising:
detecting with a nucleic acid probe for a PAX8-PPARγ1 or a PPARγ1-PAX8 nucleic acid molecule the presence of a PAX8-PPARγ1 or a PPARγ1-PAX8 molecule in the sample.
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The method of claim 1 , wherein the detecting comprises:
contacting the sample with at least two nucleic acid amplification primers, wherein a first nucleic acid amplification primer is capable of hybridizing to a PAX8 nucleic acid molecule and a second nucleic acid amplification primer is capable of hybridizing to a PPARγ1 nucleic acid molecule,
amplifying a primed nucleic acid molecule which hybridizes to the first and the second nucleic acid amplification primers, and
detecting the presence of an amplified nucleic acid molecule in the sample.
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The method of claim 1 , wherein the detecting comprises:
contacting the sample with at least two nucleic acid probes, wherein a first nucleic acid probe is capable of hybridizing to a PAX8 nucleic acid molecule and a second nucleic acid probe is capable of hybridizing to a PPARγ1 nucleic acid molecule, and
detecting the presence of a nucleic acid molecule in the sample which hybridizes to both the first and the second nucleic acid probes.
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The method of claim 1 , wherein the detecting comprises:
contacting the sample with a nucleic acid probe which is capable of hybridizing to a PAX8-PPARγ1 nucleic acid fusion juncture, and
detecting the presence of a nucleic acid molecule in the sample which hybridizes to the nucleic acid probe.
- The method of claim 4 , wherein the PAX8-PPARγ1 nucleic acid fusion juncture comprises a sequence selected from the group consisting of SEQ ID NO:7, SEQ ID NO:9 and SEQ ID NO:11.
- The method of claim 1 , wherein the detecting comprises amplifying the PAX8-PPARγ1 or PPARγ1-PAX8 nucleic acid.
- The method of claim 1 , wherein the sample is a thyroid tissue sample.
- The method of claim 7 , wherein the thyroid tissue sample is from a patient suspected of having a thyroid cancer.
Owners (US)
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Brigham And Women's Hospital Inc. The
(Apr 24 2001)
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Applicants
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Brigham & Womens Hospital
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Inventors
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Fletcher Jonathan A
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Kroll Todd G
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IPC Classifications
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Document History
- Publication: Apr 20, 2004
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Application:
Jan 18, 2001
US 76511101 A
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Priority:
Jan 18, 2001
US 76511101 A
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Priority:
Aug 14, 2000
US 22507900 P
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Priority:
Jan 20, 2000
US 17710900 P