{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_6713283_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"055-494-956-927-424"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":12167,"type":"PATENT","title":"University of Oklahoma Norman Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":1965,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8665,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:Univ* Oklahoma. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1968
Search Applicants and Owners separately:Univ* Oklahoma. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1968
providing a host cell transformed or transfected with a vector comprising a polynucleotide encoding a tyrosylprotein sulfotransferase comprising amino acid residues 76-93, 96-102, 156-162, 184-188, 196-200, 202-205, 239-245 and 330-336 of SEQ ID NO:1;
culturing the host cell and causing expression of the tyrosylprotein sulfotransferase; and
purifying the tyrosylprotein sulfotransferase from the cultured host cell."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The tyrosylprotein sulfotransferase of claim 1 further defined as lacking a transmembrane domain."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["An in vitro method of sulfating a tyrosine residue on a protein, polypeptide, or peptide, comprising:
providing a protein, polypeptide or peptide comprising at least one tyrosine residue;
providing a purified tyrosylprotein sulfotransferase;
providing a sulfate donor; and
combining the protein, polypeptide, or peptide with the tyrosylprotein sulfotransferase and the sulfate donor under conditions suitable for causing transfer of the sulfate from the sulfate donor to the tyrosine of the protein, polypeptide, or peptide thereby forming a sulfated protein, polypeptide or peptide;
and wherein the tyrosylprotein sulfotransferase comprises amino acid residues 76-93, 96-102, 156-162, 184-188, 196-200, 202-205, 239-245, and 330-336 of SEQ ID No:1."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3 wherein in the step of providing a purified tyrosylprotein sulfotransferase, the tyrosylprotein sulfotransferase lacks a transmembrane domain."],"number":4,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}