{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_6709821_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"155-361-180-505-828","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10731,"type":"PATENT","title":"The University of North Carolina at Chapel Hill - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":8887,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8232,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:\"uni* north caro*\". Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1000
Search Applicants and Owners separately:\"uni* north caro*\". Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1000
(a) contacting a multivalent recognition unit complex with a plurality of polypeptides from a cDNA expression library, in which the recognition units are peptides having in the range 6 to 60 amino acid residues and which selectively bind an SH2 domain; and
(b) identifying a polypeptide having a selective binding affinity for said recognition unit complex wherein the binding specificity of the recognition units has been decreased by incorporating said recognition units into said multivalent recognition unit complex."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["A method of identifying a polypeptide comprising an SH2 domain of interest comprising:
(a) contacting a multivalent recognition unit complex, which complex comprises
(i) avidin or streptavidin, and
(ii) biotinylated recognition units, with a plurality of polypeptides from a cDNA expression library, in which the recognition units are peptides having in the range of 6 to 60 amino acid residues and which selectively binds an SH2 domain; and
(b) identifying a polypeptide having a selective binding affinity for said recognition unit complex;wherein the binding specificity of the recognition units has been decreased by incorporating said recognition units into said multivalent recognition unit complex."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 , wherein the multivalent recognition unit complex comprises a complex selected from the group consisting of: (a) biotinylated recognition units and avidin or streptavidin, (b) recognition units in the form of multiple antigenic peptides, or (c) recognition units cross-linked to a carrier protein."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of any one of claim 1 , 2 , or 3 in which of polypeptides is obtained from a virus."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of any one of claim 1 , 2 , or 3 in which said expression library is a recombinant bacteriophage library."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 5 in which said expression library is a recombinant M13 library."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of any one of claim 1 , 2 , or 3 in which said expression library is a recombinant plasmid or cosmid library."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of any one of claim 1 , 2 , or 3 in which said recognition unit is a peptide having 20 to 50 amino acid residues."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 or claim 3 in which the valency of the recognition unit in the complex is at least four."],"number":9,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}