System For Efficient Secretion Of Recombinant Proteins

Abstract

Provided is a signal peptide comprising an amino acid sequence at least 90% homologous to the amino acid sequence as shown in SEQ ID NO:1. Further provided is a fusion protein comprising the signal peptide fused to a heterologous protein. Also provided are nucleic acid molecules encoding the signal peptide and encoding the fusion protein, as well as vectors and recombinant host cells comprising the nucleic acid molecules. The recombinant host cell can be a recombinant bacterium having a functional type III secretion system and having loss-of-function mutations in genes that encode secreted substrate proteins of the type III secretion system. The recombinant host cell can be used in a method of producing the heterologous protein.


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Document History
  • Publication: Dec 16, 2003
  • Application: Apr 8, 1999
    US US 28843899 A
  • Priority: Apr 8, 1999
    US US 28843899 A

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