{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_6586411_B1","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"100-652-627-030-908","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11624,"type":"PATENT","title":"Mayo Medical School - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":5667,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8299,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: mayo medi*
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Select all patents in the collection and expand by simple families. Add to collection. Total patents: 5671
Search Applicants and Owners separately: mayo medi*
Select more for logical variants. Add to collection.
Select all patents in the collection and expand by simple families. Add to collection. Total patents: 5671
administering to a mammal in need thereof nucleic acid comprising the transgene and a sequence encoding a sodium-iodide symporter (NIS), wherein the expression of said NIS sequence in cells permits cellular uptake of iodine;
administering to the mammal labeled iodine in an amount sufficient to permit transport of the labeled iodine by the NIS and detection of transported labeled iodine; and
determining the location of the transported labeled iodine in the mammal as an indication of the location of the transgene."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 , wherein the step of detecting is performed quantitatively to determine the amount of transported labeled iodine in the mammal."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["A method of monitoring the location of a transgene in a mammal, comprising the steps of:
transfecting a host cell ex vivo with nucleic acid comprising said transgene and a sequence encoding NIS, wherein expression of said NIS sequence in said host cell permits cellular uptake of iodine by said host cell;
introducing the transfected host cell into the mammal;
administering to the mammal labeled iodine in an amount sufficient to permit transport of the labeled iodine by NIS and detection of transported labeled iodine; and
determining the location of transported labeled iodine in the mammal; whereby the location of transported labeled iodine is indicative of the location of the transgene."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 or 3 , wherein the labeled iodine is radioactive iodine."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 or 3 , wherein said nucleic acid comprises a chimeric gene comprising said transgene and said sequence encoding NIS, wherein the chimeric gene also comprises a sequence encoding a protease-cleavable amino acid linker between said transgene and said sequence encoding NIS."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 5 , wherein the sequence encoding the protease-cleavable amino acid linker comprises a sequence encoding an auto-cleaving amino acid sequence."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 or 3 , wherein a first promoter is operably linked to said transgene and a second promoter is operably linked to said sequence encoding NIS."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 or 3 , wherein said nucleic acid comprises a chimeric gene comprising said transgene and said sequence encoding NIS, wherein the chimeric gene also comprises between said transgene and said sequence encoding NIS, a sequence encoding an internal ribosome entry site."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 5 , wherein the sequence encoding a protease-cleavable linker is fused in-frame to the 5′ end of a transgene."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 5 , wherein the sequence encoding a protease-cleavable linker is fused in-frame to the 3′ end of a transgene."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 5 , wherein said protease cleavable linker is cleaved by furin."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 5 , wherein said protease-cleavable linker is identical to a linker present in a cytoplasmic protein."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 or 3 , wherein said transgene encodes a fusogenic polypeptide."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 13 , wherein the fusogenic polypeptide encodes a viral fusion protein."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 13 , wherein the fusogenic polypeptide encodes a measles virus H glycoprotein."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 13 , wherein the fusogenic polypeptide encodes a gibbon ape leukemia virus envelope glycoprotein."],"number":16,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}