{"search_session":{},"preferences":{"l":"ar","queryLanguage":"ar"},"patentId":"US_6544729_B2","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"039-832-336-116-70X"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10784,"type":"PATENT","title":"Purdue University - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":7369,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8254,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: \"Purdue Uni*\";
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 467
Search Applicants and Owners separately: uni* AND Tennes* Knox*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 3052
Search Applicants and Owners separately: \"Purdue Uni*\";
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 467
Search Applicants and Owners separately: uni* AND Tennes* Knox*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 3052
(a) a genetically engineered bacterial bioluminescent bioreporter cell population responsive to an inducer;
(b) at least one bacteriophage capable of specifically infecting said bacterial target cell but not the bioreporter cell, said bacteriophage being genetically modified to cause the target cell to express said inducer upon infection of the target cell; and
(c) at least one container suitable for housing the bioreporter cell population, target cell population and bacteriophage, said container being operably connected to a light measuring device selected from the group consisting of an integrated circuit, a photomultiplier tube, a photodiode, a microchannel plate, and a charge-coupled device, said light-measuring device being adapted to detect a bioluminescent signal generated in said container upon interaction of the bioreporter cell population and the inducer, wherein detecting the bioluminescent signal is correlated with detecting the presence of the target cell."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The biosensor/bioreporter device of claim 1 wherein the genetically engineered bioluminescent bioreporter cell population contains a luxR-lux pro /lux I/luxCDABE gene construct."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The biosensor/bioreporter device of claim 1 wherein the genetically modified bacteriophage contains a luxI gene."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The biosensor/bioreporter device of claim 1 wherein the infected target cell expresses a luxI gene product that induces the bioreporter cells to produce light."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The device of claim 1 wherein the genetically modified bacteriophage comprises a plurality of genetically modified bacteriophage, each specifically infecting a different target bacterium."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The device of claim 1 wherein the target bacterium is selected from the group consisting of Salmonella, Vibrio, Pseudomonas, Escherichia, Bacillus, Clostridium, Yersinia, Shigella, Legionella, Burkholderia, Staphylococcus, Streptococcus, Proteus, Enterobacter, Mycobacterium, Campylobacter, and Listeria."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The device of claim 1 wherein the genetically engineered bioreporter cells are E. coli. "],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["A system comprising the device of claim 1 , an automatic sampling device and an analysis component for data processing."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The device of claim 1 wherein the genetically engineered bioreporter cells are selected from the group consisting of Escherichia, Pseudomonas, Vibrio, Staphylococcus, Alcaligenes, Acinetobacter, Synechococcus, Aeromonas hydrophila and Ralstonia."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The device of claim 9 wherein the bioreporter cell population is held in an encapsulation matrix."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The device of claim 10 wherein the encapsulation matrix is alginate, carrageenan, acrylic vinyl acetate copolymer, latex, polyvinyl chloride polymer, sol-gel, agar, agarose, micromachined nanoporous membranes, polydimethylsiloxane (PDMS), polyacrylamide, polyurethane/polycarbomyl sulfonate, or polyvinyl alcohol."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The device of claim 1 wherein the light measuring device is an integrated circuit."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The device of claim 12 wherein the integrated circuit comprises a photodetector and low-noise electronics."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The device of claim 1 or 12 wherein the container and the light measuring device are housed in a single unit."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["A method of detecting a selected target bacterium, comprising:
providing a genetically engineered bacterial bioluminescent bioreporter cell population responsive to an inducer;
providing a modified bacteriophage capable of specifically infecting said target bacterium but not the biorporter cells, said bacteriophage being genetically modified to cause the target bacterium to express said inducer upon infection of the target bacterium, and
contacting a sample suspected of containing said target bacterium with said bacteriophage together with a population of said bioreporter bacterial cells,
wherein a detectable bioluminescent signal is induced in said cell population subsequent to infection of the target bacterium with the bacteriophage, said detectable bioluminescent signal being correlated the presence of the target bacterium."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 15 wherein said genetically modified bioreporter cells are modified to contain a luxR-lux pro /luxl/luxCDABE gene construct and said modified bacteriophage is a luxI-genetically modified bacteriophage."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 15 wherein the selected target bacterium is selected from The group consisting of Escherichia coli, Listeria monocytogenes , Salmonella spp., Campylobacter spp., B. anthracis, B. thuringiensis and B. subtilis. "],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 15 wherein the bioreporter bacterial cells are selected from the group consisting of Escherichia, Pseudomonas, Vibrio, Staphylococcus, Alcaligenes, Acinetobacter, Synechococcus, Aeromonas hydrophila and Ralstonia."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 15 wherein the bioreporter bacterial cells are Pseudomonas fluorescens. "],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["A method of determining the amount of a selected bacterium in a sample comprising:
contacting a sample suspected of containing a selected bacterium with:
(a) a bacterium-specific bacteriophage genetically modified to cause the bacterium to express an inducer upon infection of the bacterium; and
(b) a population of bacterial bioreporter cells responsive to the inducer;
wherein the amount of the selected bacterium is determined by measuring the time required for production of a selected intensity of bioluminescent signal by the bioreporter cell population in the presence and in the absence of the selected bacterium; and relating the amount of said selected bacterium to the difference in time required for production of the selected intensity of bioluminescent signal by the bioreporter cell population in the presence and in the absence of the bacterium."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["A kit for detection of selected bacteria, comprising in suitable container form:
(a) a genetically engineered bacteriophage that harbors a luxI gene and specifically infects a selected bacterium;
(b) a bioreporter cell population genetically engineered to include a luxR-lux pro /luxI/luxCDABE gene construct, said bioreporter being incapable of infection by said bacteriophage; and
(c) instructions for use."],"number":21,"annotation":false,"title":false,"claim":true},{"lines":["A kit for detection of a target bacterial cell comprising in suitable package form:
(a) a genetically engineered bacteriophage that harbors a luxI gene and specifically infects a selected bacterium;
(b) a bioreporter cell population genetically engineered to include a luxR-lux pro /luxI/luxCDABE gene construct, said bioreporter being incapable of infection by said bacteriophage; and
(c) a device for measurement of generated light, said device comprising at least one container suitable for housing the bioreporter cell population, target cell population and bacteriophage, said container being operably connected to an integrated circuit adapted for detecting a luminescent signal produced when a bacteriophage-infected target cell induces a bioluminescent protein in the bioreporter cell population; and
(d) instructions for use."],"number":22,"annotation":false,"title":false,"claim":true},{"lines":["A composition comprising:
a bacterial bioreporter cell population genetically modified to contain a luxR-lux pro /luxI/luxCDABE gene construct; and
a luxI-modified bacteriophage selective for an identified bacterium but not said bacterial bioreporter cell population."],"number":23,"annotation":false,"title":false,"claim":true},{"lines":["The composition of claim 23 wherein the bacterial cell population is E. coli. "],"number":24,"annotation":false,"title":false,"claim":true},{"lines":["The composition of claim 23 wherein the bacteriophage is M13."],"number":25,"annotation":false,"title":false,"claim":true},{"lines":["The composition of claim 23 wherein the luxI modified bacteriophage comprises a plurality of bacteriophage, each specific for a different bacterial cell host."],"number":26,"annotation":false,"title":false,"claim":true},{"lines":["A biosensor/bioreporter device for detecting a bacterial target cell population comprising:
(a) a bacterial bioluminescent bioreporter cell population responsive to an acyl-homoserine lactone inducer, said bioreporter containing a luxR-lux pro /luxI/luxCDABE gene construct;
(b) at least one bacteriophage capable of specifically infecting the bacterial target cell but not the bioreporter cell, said bacteriophage being genetically modified to cause the target cell to express said acyl-homoserine lactone inducer upon infection of the target cell; and
(c) at least one container suitable for housing the bioreporter cell population, target cell population and bacteriophage, said container being operably connected to an integrated circuit adapted to detect a bioluminescent signal generated in said container upon interaction of the bioreporter cell population and the inducer, wherein detecting the bioluminescent signal is correlated with detecting the presence of the target cell."],"number":27,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}