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a pressure of 5 MPa, a temperature of 25° C., and a time of 40 min;\n
a pressure of 5 MPa, a temperature of 31° C., and a time of 30 min;\n
or a pressure of 5 MPa, a temperature of 37° C., and a time of 25 min."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein prior to the high pressure carbon dioxide treatment, the method further comprises: washing and suspending the target bacterium to obtain a bacteria suspension."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 4, wherein a physiological saline is used in both the washing and the suspending, and the physiological saline is a 0.85% (w/v) NaCl aqueous solution."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the target bacterium is a in exponential phase."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 6, wherein the target bacterium is Escherichia coli. "],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["(canceled)"],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 6, wherein the target bacterium is Escherichia coli O157:H7"],"number":9,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}